Application of the Reverse Line Blot Assay for the Molecular Detection of Theileria and Babesia sp in Sheep and Goat Blood Samples from Pakistan
文献类型：期刊论文
| 作者 | Iqbal F ; Khattak RM ; Ozubek S ; Khattak MNK ; Rasul A ; Aktas M |
| 刊名 | iranian journal of parasitology
 |
| 出版日期 | 2013 |
| 卷号 | 8期号:2页码:289-295 |
| 关键词 | SOUTHERN PUNJAB PAKISTAN SMALL RUMINANTS PREVALENCE CATTLE PCR ANNULATA OVIS HYBRIDIZATION INFECTION PROFILE |
| ISSN号 | 1735-7020 |
| 通讯作者 | iqbal f |
| 中文摘要 | background: the present study was designed to detect the presence of tick-borne parasites (theileria and babesia spp.) in 196 blood samples collected from apparently healthy sheep and goats from two provinces, punjab and khyber pukhtoon khwa, in pakistan. methods: reverse line blot (rlb) assay was applied for the parasitic detection by the amplification of hypervariable v4 region of the 18s ribosomal rna (rrna) gene. a membrane with covalently linked generic and species specific oligonucleotide probes was used for the hybridization of amplified pcr products. results: parasites were detected in 16% of the ruminant blood samples under study. two theileria species, t. lestoquardi and t. ovis, were identified in samples. 25, of the total 32, infected animals were from khyber pukhtoon khwa. conclusion: sheep were more prone to tick borne haemoprotozans as 81% infected samples were sheep as compared to 19% goats (p > 0.001). risk factor analysis revealed that male (p = 0.03), animals infested by ticks (p = 0.03) and herd composed of sheep only (p = 0.001) were more infected by blood parasites. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000321428700015 |
| 公开日期 | 2014-04-20 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/50486]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Iqbal F,Khattak RM,Ozubek S,et al. Application of the Reverse Line Blot Assay for the Molecular Detection of Theileria and Babesia sp in Sheep and Goat Blood Samples from Pakistan[J]. iranian journal of parasitology,2013,8(2):289-295. |
| APA | Iqbal F,Khattak RM,Ozubek S,Khattak MNK,Rasul A,&Aktas M.(2013).Application of the Reverse Line Blot Assay for the Molecular Detection of Theileria and Babesia sp in Sheep and Goat Blood Samples from Pakistan.iranian journal of parasitology,8(2),289-295. |
| MLA | Iqbal F,et al."Application of the Reverse Line Blot Assay for the Molecular Detection of Theileria and Babesia sp in Sheep and Goat Blood Samples from Pakistan".iranian journal of parasitology 8.2(2013):289-295. |
入库方式： OAI收割
来源：长春应用化学研究所
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