EXTRACTION OF LYSOZYME, ALPHA-CHYMOTRYPSIN, AND PEPSIN INTO REVERSE MICELLES FORMED USING AN ANIONIC SURFACTANT, ISOOCTANE, AND WATER
文献类型：期刊论文
| 作者 | Chang, Q. L. ; Liu, H. Z. ; Chen, J. Y. |
| 刊名 | Enzyme and Microbial Technology
 |
| 出版日期 | 1994 |
| 期号 | 11页码:970-973 |
| 关键词 | reverse micelles dynamic laser scatter extraction lysozyme alpha-chymotrypsin pepsin liquid-liquid-extraction protein extraction solubilization recovery |
| 其他题名 | Enzyme Microb. Technol. |
| 中文摘要 | The extraction of lysozyme, alpha-chymotrypsin, and pepsin from buffered salt solutions into reverse micelles was examined at different pH values and surfactant concentrations. The reverse micelles were formed by mixing aqueous buffer supplemented with KCl and an organic phase of isooctane(2,2,4-trimethylpentane), containing the anionic surfactant, Aerosol O. T. (dioctyl ester of sodium sulfosuccinic acid). The technique of dynamic laser scattering was used to measure the size of reverse micelles which were in equilibrium with the aqueous phase. It was found that the size of the reverse micelles decreased with increasing ionic strength but increased with increasing AOT concentration. In the process of extraction the reverse micelles might have rearranged themselves to host the protein. The sizes of protein-filled and -unfilled reverse micelles were different and an open equilibrium could be reached between them. Under the extraction conditions, only a small number of micelles were found to contain protein. |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| 公开日期 | 2014-03-14 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/7993]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 推荐引用方式 GB/T 7714 | Chang, Q. L.,Liu, H. Z.,Chen, J. Y.. EXTRACTION OF LYSOZYME, ALPHA-CHYMOTRYPSIN, AND PEPSIN INTO REVERSE MICELLES FORMED USING AN ANIONIC SURFACTANT, ISOOCTANE, AND WATER[J]. Enzyme and Microbial Technology,1994(11):970-973. |
| APA | Chang, Q. L.,Liu, H. Z.,&Chen, J. Y..(1994).EXTRACTION OF LYSOZYME, ALPHA-CHYMOTRYPSIN, AND PEPSIN INTO REVERSE MICELLES FORMED USING AN ANIONIC SURFACTANT, ISOOCTANE, AND WATER.Enzyme and Microbial Technology(11),970-973. |
| MLA | Chang, Q. L.,et al."EXTRACTION OF LYSOZYME, ALPHA-CHYMOTRYPSIN, AND PEPSIN INTO REVERSE MICELLES FORMED USING AN ANIONIC SURFACTANT, ISOOCTANE, AND WATER".Enzyme and Microbial Technology .11(1994):970-973. |
入库方式： OAI收割
来源：过程工程研究所
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