Analysis of mixtures of fatty acids and fatty alcohols in fermentation broth
文献类型：期刊论文
| 作者 | Liu, Yilan1,2; Chen, Ting3; Yang, Maohua1; Wang, Caixia1,2; Huo, Weiyan4; Yan, Daojiang1,2; Chen, Jinjin1,2; Zhou, Jiemin1,2; Xing, Jianmin1 |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2014-01-03 |
| 卷号 | 1323期号:3页码:66-72 |
| 关键词 | Fatty acids Fatty alcohols Fermentation broth HPLC-RID |
| ISSN号 | 0021-9673 |
| 其他题名 | J. Chromatogr. A |
| 中文摘要 | Microbial production of fatty acids and fatty alcohols has attracted increasing concerns because of energy crisis and environmental impact of fossil fuels. Therefore, simple and efficient methods for the extraction and quantification of these compounds become necessary. In this study, a high-performance liquid chromatography-refractive index detection (HPLC-RID) method was developed for the simultaneous quantification of fatty acids and fatty alcohols in these samples. The optimum chromatographic conditions are C18 column eluted with methanol:water:acetic acid (90:9.9:0.1, v/v/v); column temperature, 26 C; flow rate, 1.0 mL/min. Calibration curves of all selected analytes showed good linearity (r(2) >= 0.9989). The intra-day and inter-day relative standard deviations (RSDs) of the 10 compounds were less than 4.46% and 5.38%, respectively, which indicated that the method had good repeatability and precision. Besides, a method for simultaneous extraction of fatty acids and fatty alcohols from fermentation broth was optimized by orthogonal design. The optimal extraction conditions were as follows: solvent, ethyl acetate; solvent to sample ratio, 0.5:1; rotation speed, 2 min at 260 rpm; extraction temperature, 10 degrees C. This study provides simple and fast methods to simultaneously extract and quantify fatty acids and fatty alcohols for the first time. It will be useful for the study of microbial production of these products. (C) 2013 Elsevier B.V. All rights reserved. |
| 英文摘要 | Microbial production of fatty acids and fatty alcohols has attracted increasing concerns because of energy crisis and environmental impact of fossil fuels. Therefore, simple and efficient methods for the extraction and quantification of these compounds become necessary. In this study, a high-performance liquid chromatography-refractive index detection (HPLC-RID) method was developed for the simultaneous quantification of fatty acids and fatty alcohols in these samples. The optimum chromatographic conditions are C18 column eluted with methanol:water:acetic acid (90:9.9:0.1, v/v/v); column temperature, 26 C; flow rate, 1.0 mL/min. Calibration curves of all selected analytes showed good linearity (r(2) >= 0.9989). The intra-day and inter-day relative standard deviations (RSDs) of the 10 compounds were less than 4.46% and 5.38%, respectively, which indicated that the method had good repeatability and precision. Besides, a method for simultaneous extraction of fatty acids and fatty alcohols from fermentation broth was optimized by orthogonal design. The optimal extraction conditions were as follows: solvent, ethyl acetate; solvent to sample ratio, 0.5:1; rotation speed, 2 min at 260 rpm; extraction temperature, 10 degrees C. This study provides simple and fast methods to simultaneously extract and quantify fatty acids and fatty alcohols for the first time. It will be useful for the study of microbial production of these products. (C) 2013 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | IONIZATION MASS-SPECTROMETRY ; ESCHERICHIA-COLI ; METHYL-ESTERS ; EXTRACTION ; IDENTIFICATION ; PREDICTION ; CHEMICALS ; LEAVES ; FUELS |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000329953800008 |
| 公开日期 | 2014-05-06 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/8028]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Beijing Forestry Univ, Dept Biol Sci & Technol, Beijing 100083, Peoples R China 4.Huabei Oilfield 1 Middle Sch, Cangzhou City 062550, Hebei Province, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Yilan,Chen, Ting,Yang, Maohua,et al. Analysis of mixtures of fatty acids and fatty alcohols in fermentation broth[J]. JOURNAL OF CHROMATOGRAPHY A,2014,1323(3):66-72. |
| APA | Liu, Yilan.,Chen, Ting.,Yang, Maohua.,Wang, Caixia.,Huo, Weiyan.,...&Xing, Jianmin.(2014).Analysis of mixtures of fatty acids and fatty alcohols in fermentation broth.JOURNAL OF CHROMATOGRAPHY A,1323(3),66-72. |
| MLA | Liu, Yilan,et al."Analysis of mixtures of fatty acids and fatty alcohols in fermentation broth".JOURNAL OF CHROMATOGRAPHY A 1323.3(2014):66-72. |
入库方式： OAI收割
来源：过程工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。