beta-poly( L- malic acid) production by fed- batch culture of Aureobasidium pullulans ipe-1 with mixed sugars
文献类型：期刊论文
| 作者 | Cao, Weifeng1; Luo, Jianquan1,2; Qi, Benkun1; Zhao, Juan3; Qiao, Changsheng4; Ding, Luhui2; Su, Yi1; Wan, Yinhua1 |
| 刊名 | ENGINEERING IN LIFE SCIENCES
 |
| 出版日期 | 2014-03-01 |
| 卷号 | 14期号:2页码:180-189 |
| ISSN号 | 1618-0240 |
| 关键词 | Aureobasidium pullulans -poly (malic acid) Carbon source Fed-batch culture Mixed sugars |
| 其他题名 | Eng. Life Sci. |
| 中文摘要 | -poly(l-malic acid) (PMLA) is a biopolyester, which has attracted growing attention due to its potential applications in medicine and other industries. In this study, the biosynthetic pathway of PMLA and the fermentation strategies with mixed sugars were both investigated to enhance PMLA production by Aureobasidium pullulans ipe-1. Metabolic intermediates and inhibitors were used to study the biosynthetic pathway of PMLA. It showed that exogenous addition of l-malic acid, succinic acid, TFA, and avidin had negligible effect on PMLA production, while pyruvic acid and biotin were the inhibitors, indicating that PMLA biosynthesis was probably related to phosphoenolpyruvate via oxaloacetate catalyzed by phosphoenolpyruvate carboxylase. Sucrose was suitable for achieving the highest PMLA concentration, while fructose generated a higher yield of PMLA (PMLA produced per biomass). Furthermore, the fed-batch culture using fed solution with different sugar mixture for PMLA production was implemented. During the fed-batch culture with mixed solution, fructose could increase PMLA production. Compared with the batch culture, the feeding with mixed sugar (sucrose and glucose) increased PMLA concentration by 23.9%, up to 63.2 g/L, and the final volume of the broth was increased by 25%. These results provide a good reference for process development and optimization of PMLA production. |
| 英文摘要 | -poly(l-malic acid) (PMLA) is a biopolyester, which has attracted growing attention due to its potential applications in medicine and other industries. In this study, the biosynthetic pathway of PMLA and the fermentation strategies with mixed sugars were both investigated to enhance PMLA production by Aureobasidium pullulans ipe-1. Metabolic intermediates and inhibitors were used to study the biosynthetic pathway of PMLA. It showed that exogenous addition of l-malic acid, succinic acid, TFA, and avidin had negligible effect on PMLA production, while pyruvic acid and biotin were the inhibitors, indicating that PMLA biosynthesis was probably related to phosphoenolpyruvate via oxaloacetate catalyzed by phosphoenolpyruvate carboxylase. Sucrose was suitable for achieving the highest PMLA concentration, while fructose generated a higher yield of PMLA (PMLA produced per biomass). Furthermore, the fed-batch culture using fed solution with different sugar mixture for PMLA production was implemented. During the fed-batch culture with mixed solution, fructose could increase PMLA production. Compared with the batch culture, the feeding with mixed sugar (sucrose and glucose) increased PMLA concentration by 23.9%, up to 63.2 g/L, and the final volume of the broth was increased by 25%. These results provide a good reference for process development and optimization of PMLA production. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | FRUCTOSE PHOSPHOTRANSFERASE SYSTEM ; PHYSARUM-POLYCEPHALUM ; PHOSPHOENOLPYRUVATE CARBOXYLASE ; POLY(BETA-L-MALIC ACID) ; SALMONELLA-TYPHIMURIUM ; ESCHERICHIA-COLI ; POLY(MALIC ACID) ; POLYMALIC ACID ; CARBON-SOURCES ; DRUG-DELIVERY |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000332612300009 |
| 公开日期 | 2014-05-06 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/8035]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Technol Compiegne, Biol Engn Dept, TIMR, EA 4297, Compiegne, France 3.Tianjin Univ Sci & Technol, Res Ctr Modern Anal Technol, Tianjin, Peoples R China 4.Tianjin Univ Sci & Technol, Dept Bioengn, Tianjin, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cao, Weifeng,Luo, Jianquan,Qi, Benkun,et al. beta-poly( L- malic acid) production by fed- batch culture of Aureobasidium pullulans ipe-1 with mixed sugars[J]. ENGINEERING IN LIFE SCIENCES,2014,14(2):180-189. |
| APA | Cao, Weifeng.,Luo, Jianquan.,Qi, Benkun.,Zhao, Juan.,Qiao, Changsheng.,...&Wan, Yinhua.(2014).beta-poly( L- malic acid) production by fed- batch culture of Aureobasidium pullulans ipe-1 with mixed sugars.ENGINEERING IN LIFE SCIENCES,14(2),180-189. |
| MLA | Cao, Weifeng,et al."beta-poly( L- malic acid) production by fed- batch culture of Aureobasidium pullulans ipe-1 with mixed sugars".ENGINEERING IN LIFE SCIENCES 14.2(2014):180-189. |
入库方式： OAI收割
来源：过程工程研究所
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