Comparison of PLA Microparticles and Alum as Adjuvants for H5N1 Influenza Split Vaccine: Adjuvanticity Evaluation and Preliminary Action Mode Analysis
文献类型：期刊论文
| 作者 | Zhang, Weifeng1,2; Wang, Lianyan1; Liu, Yuan1,2; Chen, Xiaoming1,2; Li, Jiahui1; Yang, Tingyuan1; An, Wenqi3; Ma, Xiaowei3; Pan, Ruowen3; Ma, Guanghui1 |
| 刊名 | PHARMACEUTICAL RESEARCH
 |
| 出版日期 | 2014-04-01 |
| 卷号 | 31期号:4页码:1015-1031 |
| 关键词 | adjuvanticity alum influenza vaccine microparticles mode of action |
| ISSN号 | 0724-8741 |
| 其他题名 | Pharm. Res. |
| 中文摘要 | To compare the adjuvanticity of polymeric particles (new-generation adjuvant) and alum (the traditional and FDA-approved adjuvant) for H5N1 influenza split vaccine, and to investigate respective action mode. Vaccine formulations were prepared by incubating lyophilized poly(lactic acid) (PLA) microparticles or alum within antigen solution. Antigen-specific immune responses in mice were evaluated using ELISA, ELISpot, and flow cytometry assay. Adjuvants' action modes were investigated by determining antigen persistence at injection sites, local inflammation response, antigen transport into draining lymph node, and activation of DCs in secondary lymphoid organs (SLOs). Alum promoted antigen-specific humoral immune response. PLA microparticles augmented both humoral immune response and cell-mediated-immunity which might enhance cross-protection of influenza vaccine. With regard to action mode, alum adjuvant functions by improving antigen persistence at injection sites, inducing severe local inflammation, slightly improving antigen transport into draining lymph nodes, and improving the expression of MHC II on DCs in SLOs. PLA microparticles function by slightly improving antigen transport into draining lymph nodes, and promoting the expression of both MHC molecules and co-stimulatory molecules on DCs in SLOs. Considering the adjuvanticity and side effects (local inflammation) of both adjuvants, we conclude that PLA microparticles are promising alternative adjuvant for H5N1 influenza split vaccine. |
| 英文摘要 | To compare the adjuvanticity of polymeric particles (new-generation adjuvant) and alum (the traditional and FDA-approved adjuvant) for H5N1 influenza split vaccine, and to investigate respective action mode. |
| WOS标题词 | Science & Technology ; Physical Sciences ; Life Sciences & Biomedicine |
| 类目[WOS] | Chemistry, Multidisciplinary ; Pharmacology & Pharmacy |
| 研究领域[WOS] | Chemistry ; Pharmacology & Pharmacy |
| 关键词[WOS] | VIRUS-LIKE PARTICLE ; IMMUNE-RESPONSES ; DELIVERY-SYSTEMS ; CELL DIFFERENTIATION ; CROSS-PRESENTATION ; DENDRITIC CELLS ; NANOPARTICLES ; ANTIGEN ; PROTECTION ; RECEPTORS |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000333080100015 |
| 公开日期 | 2014-05-06 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/8058]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Natl Key Lab Biochem Engn, PLA Key Lab Biopharmaceut Prod & Formulat Engn, Inst Proc Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Hualan Biol Engn Inc, Xinxiang 453003, Henan Province, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Weifeng,Wang, Lianyan,Liu, Yuan,et al. Comparison of PLA Microparticles and Alum as Adjuvants for H5N1 Influenza Split Vaccine: Adjuvanticity Evaluation and Preliminary Action Mode Analysis[J]. PHARMACEUTICAL RESEARCH,2014,31(4):1015-1031. |
| APA | Zhang, Weifeng.,Wang, Lianyan.,Liu, Yuan.,Chen, Xiaoming.,Li, Jiahui.,...&Ma, Guanghui.(2014).Comparison of PLA Microparticles and Alum as Adjuvants for H5N1 Influenza Split Vaccine: Adjuvanticity Evaluation and Preliminary Action Mode Analysis.PHARMACEUTICAL RESEARCH,31(4),1015-1031. |
| MLA | Zhang, Weifeng,et al."Comparison of PLA Microparticles and Alum as Adjuvants for H5N1 Influenza Split Vaccine: Adjuvanticity Evaluation and Preliminary Action Mode Analysis".PHARMACEUTICAL RESEARCH 31.4(2014):1015-1031. |
入库方式： OAI收割
来源：过程工程研究所
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