Development of a mixed solvent system for the efficient resolution of (R, S)-2-octanol catalyzed by magnetite-immobilized lipase
文献类型：期刊论文
| 作者 | Liu, Ying1; Guo, Chen1,2; Liu, Chun-Zhao1 |
| 刊名 | JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
 |
| 出版日期 | 2014-03-01 |
| 卷号 | 101期号:1页码:23-27 |
| 关键词 | Yarrowia lipolytica lipase Resolution 2-Octanol Enantioselectivity Magnetite |
| ISSN号 | 1381-1177 |
| 其他题名 | J. Mol. Catal. B-Enzym. |
| 中文摘要 | In order to find a suitable reaction system for the enzymatic resolution of (R, S)-2-octanol, the effects of the molecular structure of the solvent on the enantioselectivity (E) and enzymatic activity of Yarrowia lipolytica lipase (YLL) immobilized onto magnetic nanoparticles were systematically analyzed. Both the E and enzymatic activity of the reaction in an acyclic, structurally linear solvent were higher than those in the corresponding branched chain solvent or cyclic solvent. In a mixed solvent system with acetone and carbon tetrachloride (v/v = 3:7), the immobilized YLL exhibited high enantioselectivity, activity, and reusability. The thermodynamic analysis showed that the enantiomer discrimination was enthalpy-driven at all temperatures tested. These results present new opportunities and challenges for understanding and intensifying the enzymatic resolution process of (R, S)-2-octanol by designing suitable solvent system. (C) 2013 Elsevier B.V. All rights reserved. |
| 英文摘要 | In order to find a suitable reaction system for the enzymatic resolution of (R, S)-2-octanol, the effects of the molecular structure of the solvent on the enantioselectivity (E) and enzymatic activity of Yarrowia lipolytica lipase (YLL) immobilized onto magnetic nanoparticles were systematically analyzed. Both the E and enzymatic activity of the reaction in an acyclic, structurally linear solvent were higher than those in the corresponding branched chain solvent or cyclic solvent. In a mixed solvent system with acetone and carbon tetrachloride (v/v = 3:7), the immobilized YLL exhibited high enantioselectivity, activity, and reusability. The thermodynamic analysis showed that the enantiomer discrimination was enthalpy-driven at all temperatures tested. These results present new opportunities and challenges for understanding and intensifying the enzymatic resolution process of (R, S)-2-octanol by designing suitable solvent system. (C) 2013 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemistry & Molecular Biology ; Chemistry, Physical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | ORGANIC-SOLVENTS ; SUBSTRATE-SPECIFICITY ; KINETIC RESOLUTION ; ENANTIOSELECTIVITY ; WATER ; ENZYMES ; MEDIA ; TRANSESTERIFICATION ; BIOCATALYSIS ; PERFORMANCE |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000332142400004 |
| 公开日期 | 2014-05-06 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/8072]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Ying,Guo, Chen,Liu, Chun-Zhao. Development of a mixed solvent system for the efficient resolution of (R, S)-2-octanol catalyzed by magnetite-immobilized lipase[J]. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,2014,101(1):23-27. |
| APA | Liu, Ying,Guo, Chen,&Liu, Chun-Zhao.(2014).Development of a mixed solvent system for the efficient resolution of (R, S)-2-octanol catalyzed by magnetite-immobilized lipase.JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,101(1),23-27. |
| MLA | Liu, Ying,et al."Development of a mixed solvent system for the efficient resolution of (R, S)-2-octanol catalyzed by magnetite-immobilized lipase".JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 101.1(2014):23-27. |
入库方式： OAI收割
来源：过程工程研究所
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