A Microfluidic-Based Multi-Shear Device for Investigating the Effects of Low Fluid-Induced Stresses on Osteoblasts
文献类型：期刊论文
| 作者 | Yu, WL; Qu, H; Hu GQ(胡国庆) ; Zhang, Q; Song, K; Guan, HJ; Liu, TJ; Qin, JH
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| 刊名 | PLOS ONE
 |
| 出版日期 | 2014-02-27 |
| 卷号 | 9期号:2页码:e89966 |
| 通讯作者邮箱 | tingjiao@dlmedu.edu.cn; jhqin@dicp.ac.cn |
| 关键词 | elasto-plastic-damage behavior anisotropy physical mechanism finite deformation constitutive theory component assembling model |
| ISSN号 | 1932-6203 |
| 产权排序 | [Yu, Weiliang; Zhang, Qian; Guan, Haijie; Liu, Tingjiao] Dalian Med Univ, Coll Stomatol, Sect Oral Pathol, Dalian, Peoples R China; [Yu, Weiliang; Qu, Hong] Dalian Med Univ, Coll Stomatol, Sect Orthodont, Dalian, Peoples R China; [Qin, Jianhua] Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian, Peoples R China; [Hu, Guoqing; Song, Kui] Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech LNM, Beijing 100080, Peoples R China |
| 通讯作者 | Liu, TJ (reprint author), Dalian Med Univ, Coll Stomatol, Sect Oral Pathol, Dalian, Peoples R China. |
| 合作状况 | 国内 |
| 中文摘要 | Interstitial fluid flow (IFF) within the extracellular matrix (ECM) produces low magnitude shear stresses on cells. Fluid flow-induced stress (FSS) plays an important role during tissue morphogenesis. To investigate the effect of low FSS generated by IFF on cells, we developed a microfluidic-based cell culture device that can generate multiple low shear stresses. By changing the length and width of the flow-in channels, different continuous low level shear stresses could be generated in individual cell culture chambers. Numerical calculations demonstrate uniform shear stress distributions of the major cell culture area of each chamber. This calculation is further confirmed by the wall shear stress curves. The effects of low FSS on MC3T3-E1 proliferation and differentiation were studied using this device. It was found that FSS ranging from 1.5 to 52.6 mu Pa promoted MC3T3-E1 proliferation and differentiation, but FSS over 412 mu Pa inhibited the proliferation and differentiation of MC3T3-E1 cells. FSS ranging from 1.5 to 52.6 mu Pa also increased the expression of Runx2, a key transcription factor regulating osteoblast differentiation. It is suggested that Runx2 might be an important regulator in low FSS-induced MC3T3-E1 differentiation. This device allows for detailed study of the effect of low FSS on the behaviors of cells; thus, it would be a useful tool for analysis of the effects of IFF-induced shear stresses on cells. |
| 学科主题 | Science & Technology - Other Topics |
| 分类号 | 二类/Q1 |
| 收录类别 | SCI |
| 资助信息 | This work was supported by National Natural Science Foundation of China (No. 81171425) and Program for Liaoning Excellent Talents in University (No. LR201013). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. |
| 原文出处 | http://dx.doi.org/10.1371/journal.pone.0089966 |
| 语种 | 英语 |
| WOS记录号 | WOS:000332390800061 |
| 公开日期 | 2014-05-09 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/48765]  |
| 专题 | 力学研究所_非线性力学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Yu, WL,Qu, H,Hu GQ,et al. A Microfluidic-Based Multi-Shear Device for Investigating the Effects of Low Fluid-Induced Stresses on Osteoblasts[J]. PLOS ONE,2014,9(2):e89966. |
| APA | Yu, WL.,Qu, H.,Hu GQ.,Zhang, Q.,Song, K.,...&Qin, JH.(2014).A Microfluidic-Based Multi-Shear Device for Investigating the Effects of Low Fluid-Induced Stresses on Osteoblasts.PLOS ONE,9(2),e89966. |
| MLA | Yu, WL,et al."A Microfluidic-Based Multi-Shear Device for Investigating the Effects of Low Fluid-Induced Stresses on Osteoblasts".PLOS ONE 9.2(2014):e89966. |
入库方式： OAI收割
来源：力学研究所
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