Kinetic behaviour of the cells touching substrate: the interfacial stiffness guides cell spreading
文献类型：期刊论文
| 作者 | Li JJ; Han D ; Zhao YP(赵亚溥)
|
| 刊名 | Scientific Reports
 |
| 出版日期 | 2014-01-28 |
| 卷号 | 4页码:3910 |
| 通讯作者邮箱 | dhan@nanoctr.cn; yzhao@imech.ac.cn |
| ISSN号 | 2045-2322 |
| 产权排序 | [Li, Jianjun; Zhao, Ya-Pu] Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech, Beijing 100190, Peoples R China; [Han, Dong] Natl Ctr Nanosci & Technol, Beijing 100190, Peoples R China |
| 通讯作者 | Zhao, YP (reprint author), Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech, Beijing 100190, Peoples R China. |
| 合作状况 | 国内 |
| 中文摘要 | To describe detailed behaviour of cell spreading under the influence of substrate stiffness, A549 cells cultured on the surfaces of polydimethylsiloxane (PDMS) and polyacrylamide (PAAm) with bulk rigidities ranging from 0.1 kPa to 40 kPa were in situ observed. The spreading behaviour of cells on PAAm presented a positive correlation between spreading speed and substrate stiffness. After computing the deformations of PAAm gels and collagen, the bulk stiffness of PAAm, rather than matrix tethering, determined the cell behaviour. On the other hand, spreading behaviour of the cells was unaffected by varying the bulk stiffness of PDMS. Based on simulation analyses, the elasticity of silica-like layer induced by UV radiation on PDMS surface dominated cell-substrate interaction, rather than the bulk stiffness of the material, indicating that it is the interfacial stiffness that mainly guided the cell spreading. And then the kinetics of cell spreading was for the first time modeled based on absolute rate theory. |
| 学科主题 | Science & Technology - Other Topics |
| 分类号 | 一类 |
| 收录类别 | SCI |
| 资助信息 | This work was jointly supported by the National Natural Science Foundation of China (NSFC, Grant No. 11072244), the Key Research Program of the Chinese Academy of Sciences (Grant No. KJZD-EW-M01), the Instrument Developing Project of the Chinese Academy of Sciences (Grant No. Y2010031) and the project of 973 in Ministry of Science and Technology of China (Grant No. 2012CB933800). |
| 原文出处 | http://dx.doi.org/10.1038/srep03910 |
| 语种 | 英语 |
| WOS记录号 | WOS:000331152700004 |
| 公开日期 | 2014-05-09 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/48779]  |
| 专题 | 力学研究所_非线性力学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Li JJ,Han D,Zhao YP. Kinetic behaviour of the cells touching substrate: the interfacial stiffness guides cell spreading[J]. Scientific Reports,2014,4:3910. |
| APA | Li JJ,Han D,&Zhao YP.(2014).Kinetic behaviour of the cells touching substrate: the interfacial stiffness guides cell spreading.Scientific Reports,4,3910. |
| MLA | Li JJ,et al."Kinetic behaviour of the cells touching substrate: the interfacial stiffness guides cell spreading".Scientific Reports 4(2014):3910. |
入库方式： OAI收割
来源：力学研究所
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