VEGF(189) Expression Is Highly Related to Adaptation of the Plateau Pika (Ochotona curzoniae) Inhabiting High Altitudes
文献类型：期刊论文
| 作者 | Li, Hongge1,2; Guo, Songchang1; Ren, Yongming3; Wang, Depeng1,2; Yu, Honghao1,2; Li, Wenjing1,2; Zhao, Xinquan1; Chang, Zhijie3 |
| 刊名 | high altitude medicine & biology
 |
| 出版日期 | 2013-12-01 |
| 卷号 | 14期号:4页码:395-404 |
| 关键词 | adaptation Qinghai-Tibetan plateau hypoxia plateau pika VEGF real-time PCR |
| ISSN号 | 1527-0297 |
| 通讯作者 | zhao, xq (reprint author), chinese acad sci, northwest inst plateau biol, xining 810001, peoples r china. |
| 中文摘要 | li, hongge, songchang guo, yongming ren, depeng wang, honghao yu, wenjing li, xinquan zhao, and zhijie chang. vegf(189) expression is highly related to adaptation of the plateau pika (ochotona curzoniae) inhabiting high altitudes. high alt med biol 14:395-404, 2013. the plateau pika (ochotona curzonia) has adapted to high-altitude hypoxia during evolution. higher microvessel density in specific tissues and a blunted hypoxic pulmonary vasoconstriction response are the critical components of this adaptation. vegf, vascular endothelial growth factor, has proved to be a key regulator of angiogenesis in response to tissue hypoxia and to play an important role in vascular vasodilation. however, the role of vegf in adaptation to high-altitude hypoxia in the plateau pika remains unknown. in this study, we cloned cdnas for vegf(165) and vegf(189) and examined their expression in pikas inhabiting altitudes of 3200 and 4750m. phylogenetic analysis reveals that pika vegf(165) and vegf(189) are evolutionarily conserved. real-time pcr analysis demonstrates that vegf(165) and vegf(189) display tissue and altitude-specific expression patterns. interestingly, we found that the levels of vegf(189) mrna are significantly higher than those of vegf(165) in the brain and muscle tissues of the pika, which is different from what was previously observed in sea-level mammals. vegf(189) mrna levels in brain, muscle, and lung of the pika increased with increased habitat altitude, whereas vegf(165) shows less change. our study suggests an important role for vegf(189) in adaptation to hypoxia by the plateau pika in the high-altitude environment. |
| 英文摘要 | li, hongge, songchang guo, yongming ren, depeng wang, honghao yu, wenjing li, xinquan zhao, and zhijie chang. vegf(189) expression is highly related to adaptation of the plateau pika (ochotona curzoniae) inhabiting high altitudes. high alt med biol 14:395-404, 2013. the plateau pika (ochotona curzonia) has adapted to high-altitude hypoxia during evolution. higher microvessel density in specific tissues and a blunted hypoxic pulmonary vasoconstriction response are the critical components of this adaptation. vegf, vascular endothelial growth factor, has proved to be a key regulator of angiogenesis in response to tissue hypoxia and to play an important role in vascular vasodilation. however, the role of vegf in adaptation to high-altitude hypoxia in the plateau pika remains unknown. in this study, we cloned cdnas for vegf(165) and vegf(189) and examined their expression in pikas inhabiting altitudes of 3200 and 4750m. phylogenetic analysis reveals that pika vegf(165) and vegf(189) are evolutionarily conserved. real-time pcr analysis demonstrates that vegf(165) and vegf(189) display tissue and altitude-specific expression patterns. interestingly, we found that the levels of vegf(189) mrna are significantly higher than those of vegf(165) in the brain and muscle tissues of the pika, which is different from what was previously observed in sea-level mammals. vegf(189) mrna levels in brain, muscle, and lung of the pika increased with increased habitat altitude, whereas vegf(165) shows less change. our study suggests an important role for vegf(189) in adaptation to hypoxia by the plateau pika in the high-altitude environment. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biophysics ; public, environmental & occupational health ; sport sciences |
| 研究领域[WOS] | biophysics ; public, environmental & occupational health ; sport sciences |
| 关键词[WOS] | endothelial growth-factor ; zokor myospalax-baileyi ; factor messenger-rna ; hypoxia-inducible factor-1-alpha ; differential expression ; pulmonary-circulation ; systemic hypoxia ; vegf isoforms ; protein ; cells |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000329162900015 |
| 公开日期 | 2014-05-09 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/3891]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China 3.Tsinghua Univ, Sch Med, Dept Biol Sci & Biotechnol, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100084, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Hongge,Guo, Songchang,Ren, Yongming,et al. VEGF(189) Expression Is Highly Related to Adaptation of the Plateau Pika (Ochotona curzoniae) Inhabiting High Altitudes[J]. high altitude medicine & biology,2013,14(4):395-404. |
| APA | Li, Hongge.,Guo, Songchang.,Ren, Yongming.,Wang, Depeng.,Yu, Honghao.,...&Chang, Zhijie.(2013).VEGF(189) Expression Is Highly Related to Adaptation of the Plateau Pika (Ochotona curzoniae) Inhabiting High Altitudes.high altitude medicine & biology,14(4),395-404. |
| MLA | Li, Hongge,et al."VEGF(189) Expression Is Highly Related to Adaptation of the Plateau Pika (Ochotona curzoniae) Inhabiting High Altitudes".high altitude medicine & biology 14.4(2013):395-404. |
入库方式： OAI收割
来源：西北高原生物研究所
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