One-step Separation and Purification of Three Lignans and One Flavonol from Sinopodophyllum emodi by Medium-pressure Liquid Chromatography and High-speed Counter-current Chromatography
文献类型：期刊论文
| 作者 | Wang, Ping1,2; Liu, Yongling1,2; Chen, Tao1; Xu, Wenhua1 ; You, Jinmao1; Liu, Yongjun1; Li, Yulin1
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| 刊名 | phytochemical analysis
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| 出版日期 | 2013-11-01 |
| 卷号 | 24期号:6页码:603-607 |
| 关键词 | high-speed counter-current chromatography medium-pressure liquid chromatography one-step 4 '-demethylpodophyllotoxin podophyllotoxin deoxypodophyllotoxin kaempferol Sinopodophyllum emodi |
| ISSN号 | 0958-0344 |
| 通讯作者 | li, yl (reprint author), chinese acad sci, northwest inst plateau biol, key lab adaptat & evolut plateau biota, xining 810001, peoples r china. |
| 中文摘要 | introductionlignans and flavonols are the primary constituents of sinopodophyllum emodi and have been used as cathartic, anthelmintic, chemotherapeutic and anti-hypertensive compounds. although these compounds have been isolated, there have been no reports on the separation of 4-demethyl podophyllotoxin, podophyllotoxin, deoxypodophyllotoxin and kaempferol in one step by medium-pressure liquid chromatography (mplc) and high-speed counter-current chromatography (hsccc). objectivedevelopment of an efficient method for the preparative separation and purification of three lignans and one flavonol from s. emodi. methodsthe precipitate of crude extracts was first separated by mplc into four parts, numbered gj-1, gj-2, gj-3 and gj-4. gj-1 was separated and purified by hsccc using a solvent system composed of n-hexane:ethyl acetate:methanol:water (1.75:1.5:1:0.75, v/v/v/v). the purities of the target compounds were assessed using high-performance liquid chromatography (hplc) and chemical structures were identified by h-1-nmr and c-13-nmr. resultsthe hsccc and mplc methods were successfully used for the preparative separation and purification of 4-demethyl podophyllotoxin (8.5 mg, 92.4%), podophyllotoxin (40.1 mg, 92.1%), deoxypodophyllotoxin (4.6 mg, 98.1%), and kaempferol (1.6 mg, 96.7%) from a 100 mg sample. conclusionthree lignans (4-demethyl podophyllotoxin, podophyllotoxin, deoxypodophyllotoxin) and one flavonol (kaempferol) were successfully isolated by hsccc and mplc in one step. copyright (c) 2013 john wiley & sons, ltd. lignans and flavonols are the primary constituents of sinopodophyllum emodi and have been used as cathartic, anthelmintic, chemotherapeutic and anti-hypertensive compounds. in this paper, three lignans and one flavonol, including 4-demethyl podophyllotoxin, podophyllotoxin, deoxypodophyllotoxin and kaempferol, were firstly enriched by medium-pressure liquid chromatography(mplc) and then separated by high-speed counter-current chromatography(hsccc) in one step using a solvent system composed of n-hexane: ethyl acetate: methanol: water (1.75:1.5:1:0.75, v/v/v/v). |
| 英文摘要 | introductionlignans and flavonols are the primary constituents of sinopodophyllum emodi and have been used as cathartic, anthelmintic, chemotherapeutic and anti-hypertensive compounds. although these compounds have been isolated, there have been no reports on the separation of 4-demethyl podophyllotoxin, podophyllotoxin, deoxypodophyllotoxin and kaempferol in one step by medium-pressure liquid chromatography (mplc) and high-speed counter-current chromatography (hsccc). |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; plant sciences ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; plant sciences ; chemistry |
| 关键词[WOS] | preparative separation ; podophyllotoxin |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000326032100012 |
| 公开日期 | 2014-05-09 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/3910]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Ping,Liu, Yongling,Chen, Tao,et al. One-step Separation and Purification of Three Lignans and One Flavonol from Sinopodophyllum emodi by Medium-pressure Liquid Chromatography and High-speed Counter-current Chromatography[J]. phytochemical analysis,2013,24(6):603-607. |
| APA | Wang, Ping.,Liu, Yongling.,Chen, Tao.,Xu, Wenhua.,You, Jinmao.,...&Li, Yulin.(2013).One-step Separation and Purification of Three Lignans and One Flavonol from Sinopodophyllum emodi by Medium-pressure Liquid Chromatography and High-speed Counter-current Chromatography.phytochemical analysis,24(6),603-607. |
| MLA | Wang, Ping,et al."One-step Separation and Purification of Three Lignans and One Flavonol from Sinopodophyllum emodi by Medium-pressure Liquid Chromatography and High-speed Counter-current Chromatography".phytochemical analysis 24.6(2013):603-607. |
入库方式： OAI收割
来源：西北高原生物研究所
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