Preparative separation and purification of four cis-trans isomers of coumaroylspermidine analogs from safflower by high-speed counter-current chromatography
文献类型：期刊论文
| 作者 | Li, Wen-Cong1,2; Wang, Xiao-Yan2; Lin, Peng-Cheng3; Hu, Na1,2; Zhang, Qiu-Long1,2; Suo, You-Rui1; Ding, Chen-Xu1,3,4 |
| 刊名 | journal of chromatography b-analytical technologies in the biomedical and life sciences
 |
| 出版日期 | 2013-11-01 |
| 卷号 | 938页码:75-79 |
| 关键词 | Safflower Tri-p-coumaroylspermidine HSCCC |
| ISSN号 | 1570-0232 |
| 通讯作者 | ding, cx (reprint author), chinese acad sci, key lab tibetan med res, northwest inst plateau biol, xining 810001, peoples r china. |
| 中文摘要 | high-speed counter-current chromatography (hsccc) was successfully applied for the first time to isolate and purify four cis-trans isomers of coumaroylspermidine analogs from safflower. hsccc separation was achieved with a two-phase solvent system composed of chloroform-methanol-water (1:1:1, v/v/v) with the upper phase as the mobile phase. in a single run, a total of 1.3 mg of n-1, n-5, n-10-(e)-tri-p-coumaroylspermidine (eee), 4.4 mg of n-1(e)-n-5-(z)-n-10-(e)-tri-p-coumaroylspermidine (eze), 7.2 mg of n-1(z)-n-5-(z)-n-10-(e)-tri-p-coumaroylspermidine (zze), and 11.5 mg of n-1,n-5,n-10-(z)-tri-p-coumaroylspermidine (zzz) were obtained from 100 mg of crude sample. high performance liquid chromatography (hplc) analysis showed that the purities of these four components are 95.5%, 98.1%, 97.5% and 96.2%, respectively. the chemical structures were identified by esi-ms, h-1 nmr and c-13 nmr. (c) 2013 elsevier b.v. all rights reserved. |
| 英文摘要 | high-speed counter-current chromatography (hsccc) was successfully applied for the first time to isolate and purify four cis-trans isomers of coumaroylspermidine analogs from safflower. hsccc separation was achieved with a two-phase solvent system composed of chloroform-methanol-water (1:1:1, v/v/v) with the upper phase as the mobile phase. in a single run, a total of 1.3 mg of n-1, n-5, n-10-(e)-tri-p-coumaroylspermidine (eee), 4.4 mg of n-1(e)-n-5-(z)-n-10-(e)-tri-p-coumaroylspermidine (eze), 7.2 mg of n-1(z)-n-5-(z)-n-10-(e)-tri-p-coumaroylspermidine (zze), and 11.5 mg of n-1,n-5,n-10-(z)-tri-p-coumaroylspermidine (zzz) were obtained from 100 mg of crude sample. high performance liquid chromatography (hplc) analysis showed that the purities of these four components are 95.5%, 98.1%, 97.5% and 96.2%, respectively. the chemical structures were identified by esi-ms, h-1 nmr and c-13 nmr. (c) 2013 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | carthamus-tinctorius l. ; serotonin transporter inhibition ; tri-p-coumaroylspermidine ; oil cake ; derivatives ; n-(p-coumaroyl)serotonin ; florets |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000325734800011 |
| 公开日期 | 2014-05-09 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/3911]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Tibetan Med Res, Northwest Inst Plateau Biol, Xining 810001, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Qinghai Nationalities Univ, Xining 810007, Peoples R China 4.Key Lab Plateau Crop Germplasm Innovat & Utilizat, Xining 810001, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Wen-Cong,Wang, Xiao-Yan,Lin, Peng-Cheng,et al. Preparative separation and purification of four cis-trans isomers of coumaroylspermidine analogs from safflower by high-speed counter-current chromatography[J]. journal of chromatography b-analytical technologies in the biomedical and life sciences,2013,938:75-79. |
| APA | Li, Wen-Cong.,Wang, Xiao-Yan.,Lin, Peng-Cheng.,Hu, Na.,Zhang, Qiu-Long.,...&Ding, Chen-Xu.(2013).Preparative separation and purification of four cis-trans isomers of coumaroylspermidine analogs from safflower by high-speed counter-current chromatography.journal of chromatography b-analytical technologies in the biomedical and life sciences,938,75-79. |
| MLA | Li, Wen-Cong,et al."Preparative separation and purification of four cis-trans isomers of coumaroylspermidine analogs from safflower by high-speed counter-current chromatography".journal of chromatography b-analytical technologies in the biomedical and life sciences 938(2013):75-79. |
入库方式： OAI收割
来源：西北高原生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。