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Optimization of polysaccharides from Lycium ruthenicum fruit using RSM and its anti-oxidant activity
文献类型：期刊论文
| 作者 | Liu, Zenggen1,2 ; Dang, Jun1,2; Wang, Qilan1; Yu, Minfeng1,2; Jiang, Lei1,2; Mei, Lijuan1; Shao, Yun1; Tao, Yanduo1
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| 刊名 | international journal of biological macromolecules
 |
| 出版日期 | 2013-10-01 |
| 卷号 | 61页码:127-134 |
| 关键词 | Lycium ruthenicum Murr. Polysaccharides Antioxidant activity |
| ISSN号 | 0141-8130 |
| 通讯作者 | shao, y (reprint author), chinese acad sci, northwest inst plateau biol, 59 xiguan st, xining 810001, peoples r china. |
| 中文摘要 | dynamic microwave-assisted extraction (dmae) technique was employed for the extraction of polysaccharides from lycium ruthenicum (lrp). the extracting parameters were optimized by using three-variable-three-level box-behnken design and response surface methodology (rsm) based on the single-factor experiments. rsm analysis indicated good correspondence between experimental and predicted values. the optimum extraction parameters for the yield of polysaccharide were ratio of water to raw material 31.5 ml/g, extracting time 25.8 min and microwave power 544.0w. polysaccharide was analyzed by chemical methods and fourier-transform infrared (ft-ir). the antioxidant activities of lrp were investigated including scavenging activity of 2,2-diphenyl-1-picrylhydrazyl (dpph), hydrogen peroxide and free radicals of superoxide anion in vitro. the results of antioxidant activity exhibited lrp had the potential to be explored as novel natural antioxidant for using in functional foods or medicine. (c) 2013 elsevier b.v. all rights reserved. |
| 英文摘要 | dynamic microwave-assisted extraction (dmae) technique was employed for the extraction of polysaccharides from lycium ruthenicum (lrp). the extracting parameters were optimized by using three-variable-three-level box-behnken design and response surface methodology (rsm) based on the single-factor experiments. rsm analysis indicated good correspondence between experimental and predicted values. the optimum extraction parameters for the yield of polysaccharide were ratio of water to raw material 31.5 ml/g, extracting time 25.8 min and microwave power 544.0w. polysaccharide was analyzed by chemical methods and fourier-transform infrared (ft-ir). the antioxidant activities of lrp were investigated including scavenging activity of 2,2-diphenyl-1-picrylhydrazyl (dpph), hydrogen peroxide and free radicals of superoxide anion in vitro. the results of antioxidant activity exhibited lrp had the potential to be explored as novel natural antioxidant for using in functional foods or medicine. (c) 2013 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemistry & molecular biology |
| 研究领域[WOS] | biochemistry & molecular biology |
| 关键词[WOS] | microwave-assisted extraction ; response-surface methodology ; structural-characterization ; superoxide-dismutase ; in-vitro ; purification ; autoxidation ; derivatives ; barbarum ; protein |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000326660700019 |
| 公开日期 | 2014-05-09 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/3914]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Zenggen,Dang, Jun,Wang, Qilan,et al. Optimization of polysaccharides from Lycium ruthenicum fruit using RSM and its anti-oxidant activity[J]. international journal of biological macromolecules,2013,61:127-134. |
| APA | Liu, Zenggen.,Dang, Jun.,Wang, Qilan.,Yu, Minfeng.,Jiang, Lei.,...&Tao, Yanduo.(2013).Optimization of polysaccharides from Lycium ruthenicum fruit using RSM and its anti-oxidant activity.international journal of biological macromolecules,61,127-134. |
| MLA | Liu, Zenggen,et al."Optimization of polysaccharides from Lycium ruthenicum fruit using RSM and its anti-oxidant activity".international journal of biological macromolecules 61(2013):127-134. |
入库方式： OAI收割
来源：西北高原生物研究所
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