A sensitive high-performance liquid chromatography method with fluorescence detection for the determination of fatty acids as exemplified for Dendrobium species
文献类型：期刊论文
| 作者 | Zhang, Shijuan1,2; Song, Cuihua3; Chen, Guang1,2; Xia, Lian3; Wang, Xiaoyan1; You, Jinmao1,3 |
| 刊名 | european journal of lipid science and technology
 |
| 出版日期 | 2013-10-01 |
| 卷号 | 115期号:10页码:1155-1163 |
| 关键词 | Dendrobium species Fatty acids Fluorescence HPLC |
| ISSN号 | 1438-7697 |
| 通讯作者 | you, jm (reprint author), chinese acad sci, northwest plateau inst biol, xining 810001, peoples r china. |
| 中文摘要 | a novel high-performance liquid chromatography (hplc) method using 2-(9-oxoacridin-10(9h)-yl) acetohydrazide (oaah) as pre-column labeling reagent was applied to the analysis of fatty acids in four kinds of dendrobium species for the first time. the reaction of oaah with fatty acids could proceed easily and quickly in the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (edc) as condensing reagent within 20min. the derivatives exhibit excellent fluorescence property with excitation and emission wavelengths of 255 and 420nm, respectively. the 20 fatty acid derivatives were separated on a sb c18 reversed-phase column with gradient elution. good linear correlations were observed for all fatty acids with correlation coefficients of >0.996. when 50mg of sample was used for analysis, the detection limits at a signal-to-noise ratio of 3 were in the range of 0.10-0.42 mu g/g. free fatty acids and total fatty acids in four kinds of dendrobium species were analyzed by the developed method. the results indicated that the main fatty acids in dendrobium species were unsaturated linoleic acid (c18:2n-6) and saturated hexadecanoic acid. this work provides a useful tool for the safety assessment and quality control of dendrobium species. practical applications:dendrobium species, the stems of which can be used for medical purpose or as health foods, are precious plants in china. their significant medical effects have recently attracted increasing attention. however, there is little research on the fatty acid composition of dendrobium species. the proposed method provided a sensitive method for the hplc analysis of ffas and tfas in four kinds of dendrobium species. this is the first report of the fatty acid compositions of dendrobium species. it would be helpful for the comprehensive safety or quality assessment of dendrobium species. |
| 英文摘要 | a novel high-performance liquid chromatography (hplc) method using 2-(9-oxoacridin-10(9h)-yl) acetohydrazide (oaah) as pre-column labeling reagent was applied to the analysis of fatty acids in four kinds of dendrobium species for the first time. the reaction of oaah with fatty acids could proceed easily and quickly in the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (edc) as condensing reagent within 20min. the derivatives exhibit excellent fluorescence property with excitation and emission wavelengths of 255 and 420nm, respectively. the 20 fatty acid derivatives were separated on a sb c18 reversed-phase column with gradient elution. good linear correlations were observed for all fatty acids with correlation coefficients of >0.996. when 50mg of sample was used for analysis, the detection limits at a signal-to-noise ratio of 3 were in the range of 0.10-0.42 mu g/g. free fatty acids and total fatty acids in four kinds of dendrobium species were analyzed by the developed method. the results indicated that the main fatty acids in dendrobium species were unsaturated linoleic acid (c18:2n-6) and saturated hexadecanoic acid. this work provides a useful tool for the safety assessment and quality control of dendrobium species. practical applications:dendrobium species, the stems of which can be used for medical purpose or as health foods, are precious plants in china. their significant medical effects have recently attracted increasing attention. however, there is little research on the fatty acid composition of dendrobium species. the proposed method provided a sensitive method for the hplc analysis of ffas and tfas in four kinds of dendrobium species. this is the first report of the fatty acid compositions of dendrobium species. it would be helpful for the comprehensive safety or quality assessment of dendrobium species. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | food science & technology ; nutrition & dietetics |
| 研究领域[WOS] | food science & technology ; nutrition & dietetics |
| 关键词[WOS] | gas-chromatography ; carboxylic-acids ; omega-3-fatty-acids ; n-3 ; polysaccharides ; quantification ; identification ; huoshanense ; extraction ; reagent |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000325368100009 |
| 公开日期 | 2014-05-09 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/3915]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Qufu Normal Univ, Shandong Prov Key Lab Life Organ Anal, Qufu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Shijuan,Song, Cuihua,Chen, Guang,et al. A sensitive high-performance liquid chromatography method with fluorescence detection for the determination of fatty acids as exemplified for Dendrobium species[J]. european journal of lipid science and technology,2013,115(10):1155-1163. |
| APA | Zhang, Shijuan,Song, Cuihua,Chen, Guang,Xia, Lian,Wang, Xiaoyan,&You, Jinmao.(2013).A sensitive high-performance liquid chromatography method with fluorescence detection for the determination of fatty acids as exemplified for Dendrobium species.european journal of lipid science and technology,115(10),1155-1163. |
| MLA | Zhang, Shijuan,et al."A sensitive high-performance liquid chromatography method with fluorescence detection for the determination of fatty acids as exemplified for Dendrobium species".european journal of lipid science and technology 115.10(2013):1155-1163. |
入库方式： OAI收割
来源：西北高原生物研究所
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