Determination of the carbohydrates from Notopterygium forbesii Boiss by HPLC with fluorescence detection
文献类型：期刊论文
| 作者 | Zhang, Shijuan1,3; Li, Chunli1,3; Zhou, Guoying1; Che, Guodong1; You, Jinmao1,2; Suo, Yourui1 |
| 刊名 | carbohydrate polymers
 |
| 出版日期 | 2013-09-12 |
| 卷号 | 97期号:2页码:794-799 |
| 关键词 | Monosaccharides Polysaccharides HPLC Fluorescence detection Notopterygium forbesii Boiss |
| ISSN号 | 0144-8617 |
| 通讯作者 | you, jm (reprint author), chinese acad sci, northwest inst plateau biol, xining 810001, peoples r china. |
| 中文摘要 | a sensitive pre-column derivatization method wasdeveloped for analysis of carbohydrates by hplc with fluorescence detection. the introduction of 2-(12-benzo[b]acridin-5(12h)-yl)-acetohydrazide (baah) with excellent fluorescence property into the molecules of monosaccharides greatly enhanced the hplc sensitivity of the analytes. meanwhile, derivatization with baah also greatly increased the hydrophobicity of the monosaccharides and made them elute at increased retention times. the monosaccharides with similar properties therefore could be completely separated due to the increased interaction between the analytes and the column. component monosaccharides of the polysaccharides obtained from the roots, stems and leaves of notopterygium forbesii boiss (nf) were analyzed by the developed method. the results indicated that the polysaccharides of nf were mainly composed of d-galactose and d-glucose. this is the first systematic study of the sugar composition of the polysaccharides of nf. it will be helpful for the quality control of nf. (c) 2013 elsevier ltd. all rights reserved. |
| 英文摘要 | a sensitive pre-column derivatization method wasdeveloped for analysis of carbohydrates by hplc with fluorescence detection. the introduction of 2-(12-benzo[b]acridin-5(12h)-yl)-acetohydrazide (baah) with excellent fluorescence property into the molecules of monosaccharides greatly enhanced the hplc sensitivity of the analytes. meanwhile, derivatization with baah also greatly increased the hydrophobicity of the monosaccharides and made them elute at increased retention times. the monosaccharides with similar properties therefore could be completely separated due to the increased interaction between the analytes and the column. component monosaccharides of the polysaccharides obtained from the roots, stems and leaves of notopterygium forbesii boiss (nf) were analyzed by the developed method. the results indicated that the polysaccharides of nf were mainly composed of d-galactose and d-glucose. this is the first systematic study of the sugar composition of the polysaccharides of nf. it will be helpful for the quality control of nf. (c) 2013 elsevier ltd. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, applied ; chemistry, organic ; polymer science |
| 研究领域[WOS] | chemistry ; polymer science |
| 关键词[WOS] | performance liquid-chromatography ; polysaccharides ; derivatization ; sugars ; electrophoresis ; separation |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000323805000075 |
| 公开日期 | 2014-05-09 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/3926]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China 2.Qufu Normal Univ, Shandong Prov Key Lab Life Organ Anal, Qufu, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Shijuan,Li, Chunli,Zhou, Guoying,et al. Determination of the carbohydrates from Notopterygium forbesii Boiss by HPLC with fluorescence detection[J]. carbohydrate polymers,2013,97(2):794-799. |
| APA | Zhang, Shijuan,Li, Chunli,Zhou, Guoying,Che, Guodong,You, Jinmao,&Suo, Yourui.(2013).Determination of the carbohydrates from Notopterygium forbesii Boiss by HPLC with fluorescence detection.carbohydrate polymers,97(2),794-799. |
| MLA | Zhang, Shijuan,et al."Determination of the carbohydrates from Notopterygium forbesii Boiss by HPLC with fluorescence detection".carbohydrate polymers 97.2(2013):794-799. |
入库方式： OAI收割
来源：西北高原生物研究所
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