Separation and purification of five phenylpropanoid glycosides from Lamiophlomis rotata (Benth.) Kudo by a macroporous resin column combined with high-speed counter-current chromatography
文献类型：期刊论文
| 作者 | Yue, Hui-Lan1; Zhao, Xiao-Hui1,2; Mei, Li-Juan1; Shao, Yun1 |
| 刊名 | journal of separation science
 |
| 出版日期 | 2013-09-01 |
| 卷号 | 36期号:18页码:3123-3129 |
| 关键词 | HSCCC Lamiophlomis rotata (Benth.) Kudo Macroporous resins Phenylpropanoid glycosides Traditional Chinese medicines |
| ISSN号 | 1615-9306 |
| 通讯作者 | zhao, xh (reprint author), chinese acad sci, northwest inst plateau biol, key lab tibetan med res, xining 810001, peoples r china. |
| 中文摘要 | five phenylethanoid glycosides (phgs), forsythoside b, verbascoside, alyssonoside, isoverbascoside, and leucosceptoside b, were isolated and purified from lamiophlomis rotata (benth.) kudo by high-speed counter-current chromatography (hsccc) combined with macroporous resin (mr) column separation. in the present study, the two-phase solvent system composed of ethyl acetate/n-butanol/water (13:3:10, v/v/v) was used for hsccc separation. a total of 27 mg of forsythoside b, 41 mg of verbascoside, 29 mg of alyssonoside, 23 mg of isoverbascoside, and 13 mg of leucosceptoside b with purities of 97.7, 99.2, 99.5, 99.3, and 97.3%, respectively, were obtained in a one-step separation within 4 h from 150 mg of crude extract. the recoveries of the five phgs after mr-hsccc separation were 74.5, 76.5, 72.5, 76.4, and 77.0%, respectively. the chemical structures of all five compounds were identified by 1h and 13c nmr spectroscopy. |
| 英文摘要 | five phenylethanoid glycosides (phgs), forsythoside b, verbascoside, alyssonoside, isoverbascoside, and leucosceptoside b, were isolated and purified from lamiophlomis rotata (benth.) kudo by high-speed counter-current chromatography (hsccc) combined with macroporous resin (mr) column separation. in the present study, the two-phase solvent system composed of ethyl acetate/n-butanol/water (13:3:10, v/v/v) was used for hsccc separation. a total of 27 mg of forsythoside b, 41 mg of verbascoside, 29 mg of alyssonoside, 23 mg of isoverbascoside, and 13 mg of leucosceptoside b with purities of 97.7, 99.2, 99.5, 99.3, and 97.3%, respectively, were obtained in a one-step separation within 4 h from 150 mg of crude extract. the recoveries of the five phgs after mr-hsccc separation were 74.5, 76.5, 72.5, 76.4, and 77.0%, respectively. the chemical structures of all five compounds were identified by 1h and 13c nmr spectroscopy. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | scrophularia ningpoensis hemsley ; phenylethanoid glycosides ; preparative isolation ; ballota-nigra ; identification ; verbascoside ; roth ; root |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000324379400020 |
| 公开日期 | 2014-05-09 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/3927]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yue, Hui-Lan,Zhao, Xiao-Hui,Mei, Li-Juan,et al. Separation and purification of five phenylpropanoid glycosides from Lamiophlomis rotata (Benth.) Kudo by a macroporous resin column combined with high-speed counter-current chromatography[J]. journal of separation science,2013,36(18):3123-3129. |
| APA | Yue, Hui-Lan,Zhao, Xiao-Hui,Mei, Li-Juan,&Shao, Yun.(2013).Separation and purification of five phenylpropanoid glycosides from Lamiophlomis rotata (Benth.) Kudo by a macroporous resin column combined with high-speed counter-current chromatography.journal of separation science,36(18),3123-3129. |
| MLA | Yue, Hui-Lan,et al."Separation and purification of five phenylpropanoid glycosides from Lamiophlomis rotata (Benth.) Kudo by a macroporous resin column combined with high-speed counter-current chromatography".journal of separation science 36.18(2013):3123-3129. |
入库方式： OAI收割
来源：西北高原生物研究所
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