SEMI-PREPARATIVE SEPARATION AND PURIFICATION OF THREE FLAVONOIDS FROM PEDICULARIS LONGIFLORA VAR. TUBIFORMIS (KLOTZSCH) P. C. TSOONG BY HSCCC
文献类型：期刊论文
| 作者 | Zhao, Xiao-Hui1,2; Han, Fa1; Li, Yu-Lin1; Zhou, Guo-Ying1; Yue, Hui-Lan1 |
| 刊名 | journal of liquid chromatography & related technologies
 |
| 出版日期 | 2013-05-01 |
| 卷号 | 36期号:13页码:1751-1761 |
| 关键词 | apigenin chrysoeriol flavonoid HSCCC luteolin pedicularis longiflora var tubiformis (Klotzsch) P C Tsoong |
| ISSN号 | 1082-6076 |
| 通讯作者 | yue, hl (reprint author), chinese acad sci, northwest inst plateau biol, key lab adaptat & evolut plateau biota, xining 810001, peoples r china. |
| 中文摘要 | an effective highspeed countercurrent chromatography (hsccc) method was established for semi-preparative isolation and purification of bioactive chemical constituents from the tibetan medicinal plant pedicularis longiflora var. tubiformis (klotzsch) p. c. tsoong. with a two-phase solvent system composed of chloroformmethanolwater (8:4:5, v/v), 40mg of extract was separated to yield luteolin (12.5mg), apigenin (9.6mg), and chrysoeriol (4.8mg), with purities of 99.3, 98.2, and 98.6%, respectively, as determined by high-performance liquid chromatography (hplc). the chemical structures of these three components were identified by h-1 nmr and c-13 nmr. |
| 英文摘要 | an effective highspeed countercurrent chromatography (hsccc) method was established for semi-preparative isolation and purification of bioactive chemical constituents from the tibetan medicinal plant pedicularis longiflora var. tubiformis (klotzsch) p. c. tsoong. with a two-phase solvent system composed of chloroformmethanolwater (8:4:5, v/v), 40mg of extract was separated to yield luteolin (12.5mg), apigenin (9.6mg), and chrysoeriol (4.8mg), with purities of 99.3, 98.2, and 98.6%, respectively, as determined by high-performance liquid chromatography (hplc). the chemical structures of these three components were identified by h-1 nmr and c-13 nmr. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | counter-current chromatography ; preparative isolation ; g(2)/m arrest ; apigenin ; apoptosis ; leaves ; cells ; efficient ; pathway |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000318779800001 |
| 公开日期 | 2014-05-09 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/3946]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Xiao-Hui,Han, Fa,Li, Yu-Lin,et al. SEMI-PREPARATIVE SEPARATION AND PURIFICATION OF THREE FLAVONOIDS FROM PEDICULARIS LONGIFLORA VAR. TUBIFORMIS (KLOTZSCH) P. C. TSOONG BY HSCCC[J]. journal of liquid chromatography & related technologies,2013,36(13):1751-1761. |
| APA | Zhao, Xiao-Hui,Han, Fa,Li, Yu-Lin,Zhou, Guo-Ying,&Yue, Hui-Lan.(2013).SEMI-PREPARATIVE SEPARATION AND PURIFICATION OF THREE FLAVONOIDS FROM PEDICULARIS LONGIFLORA VAR. TUBIFORMIS (KLOTZSCH) P. C. TSOONG BY HSCCC.journal of liquid chromatography & related technologies,36(13),1751-1761. |
| MLA | Zhao, Xiao-Hui,et al."SEMI-PREPARATIVE SEPARATION AND PURIFICATION OF THREE FLAVONOIDS FROM PEDICULARIS LONGIFLORA VAR. TUBIFORMIS (KLOTZSCH) P. C. TSOONG BY HSCCC".journal of liquid chromatography & related technologies 36.13(2013):1751-1761. |
入库方式： OAI收割
来源：西北高原生物研究所
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