Culturable actinomycetes from desert ecosystem in northeast of Qinghai-Tibet Plateau
文献类型：期刊论文
| 作者 | Ding, Dong1; Chen, Guichen1; Wang, Bochan2; Wang, Qilan1; Liu, Demei1; Peng, Min1; Shi, Ping2 |
| 刊名 | annals of microbiology
 |
| 出版日期 | 2013-03-01 |
| 卷号 | 63期号:1页码:259-266 |
| 关键词 | Qinghai-Tibet Plateau Actinomycete 16S rRNA-RFLP PKS NRPS Desert ecosystem |
| ISSN号 | 1590-4261 |
| 通讯作者 | peng, m (reprint author), chinese acad sci, nw inst plateau biol, xining 810008, peoples r china. |
| 中文摘要 | in this study, 53 actinomycetes strains were isolated from desert ecosystems located in northeast of qinghai-tibet plateau and grouped into four rflp patterns. twenty-six actinomycetes with obvious morphology differences were chosen for phylogenetic diversity study and antimicrobial testing. as a result, the 16s rrna gene sequencing showed that these strains belonged to streptomyces, micromonospora, saccharothrix, streptosporangium and cellulomonas, and that most of the strains had antimicrobial bioactivity. the pks and nrps genes detection indicated diversified potential bioactive products of actinomycetes in this ecosystem. among these strains, sd-31 was chosen to study the bioactive products using hplc-ms because of its optimum antimicrobial bioactivity. the result showed that it might produce granatomycin a, granatomycin c, and an unknown compound. |
| 英文摘要 | in this study, 53 actinomycetes strains were isolated from desert ecosystems located in northeast of qinghai-tibet plateau and grouped into four rflp patterns. twenty-six actinomycetes with obvious morphology differences were chosen for phylogenetic diversity study and antimicrobial testing. as a result, the 16s rrna gene sequencing showed that these strains belonged to streptomyces, micromonospora, saccharothrix, streptosporangium and cellulomonas, and that most of the strains had antimicrobial bioactivity. the pks and nrps genes detection indicated diversified potential bioactive products of actinomycetes in this ecosystem. among these strains, sd-31 was chosen to study the bioactive products using hplc-ms because of its optimum antimicrobial bioactivity. the result showed that it might produce granatomycin a, granatomycin c, and an unknown compound. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biotechnology & applied microbiology ; microbiology |
| 研究领域[WOS] | biotechnology & applied microbiology ; microbiology |
| 关键词[WOS] | 16s ribosomal-rna ; sp nov. ; streptomyces-lateritius ; community composition ; rapid identification ; soil ; diversity ; amplification ; purification ; antibiotics |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000314692400028 |
| 公开日期 | 2014-05-09 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/3957]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Inst Plateau Biol, Xining 810008, Peoples R China 2.E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ding, Dong,Chen, Guichen,Wang, Bochan,et al. Culturable actinomycetes from desert ecosystem in northeast of Qinghai-Tibet Plateau[J]. annals of microbiology,2013,63(1):259-266. |
| APA | Ding, Dong.,Chen, Guichen.,Wang, Bochan.,Wang, Qilan.,Liu, Demei.,...&Shi, Ping.(2013).Culturable actinomycetes from desert ecosystem in northeast of Qinghai-Tibet Plateau.annals of microbiology,63(1),259-266. |
| MLA | Ding, Dong,et al."Culturable actinomycetes from desert ecosystem in northeast of Qinghai-Tibet Plateau".annals of microbiology 63.1(2013):259-266. |
入库方式： OAI收割
来源：西北高原生物研究所
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