Variation and their relationship of NAM-G1 gene and grain protein content in Triticum timopheevii Zhuk
文献类型：期刊论文
| 作者 | Hu, Xi-Gui1,2; Wu, Bi-Hua1,2; Liu, Deng-Cai1,2,3; Wei, Yu-Ming1,2; Gao, Shi-Bin2,4; Zhen, You-Liang1,2 |
| 刊名 | journal of plant physiology
 |
| 出版日期 | 2013-02-15 |
| 卷号 | 170期号:3页码:330-337 |
| 关键词 | T. timopheevii NAM-G1 gene Grain protein content |
| ISSN号 | 0176-1617 |
| 通讯作者 | wu, bh (reprint author), sichuan agr univ, triticeae res inst, wenjiang 611130, peoples r china. |
| 中文摘要 | nam is an important domestication gene and valuable to enhance grain protein contents (gpcs) of modern wheat cultivars. in the present study, 12 nam-g1 genes in triticum timopheevii zhuk. (aagg, 2n = 4x = 28) were cloned. these genes had the same length of 1546 bp including two introns and three exons, and encoded a polypeptide of 407 amino acid residues which contained a n-terminal nac domain with five sub-domains, and a c-terminal transcriptional activation region (tar). they were highly similar to the previously published functional nam-b1 gene dq871219 from t. turgidum ssp. dicoccoides korn. (aabb, 2n = 4x = 28) in both the nucleotide and protein sequence's, with a very high identity of 99.5%. the differences among the 12 nam-g1 genes resulted from 17 snps including 14 transitions and 3 transversions. they had outstandingly different expression levels in qrt-pcr. and, their relative expression quantities were significantly positively correlated with gpc of the accessions. in addition, the difference in amino acid sequences of the nam-g1 genes may also affect the gpc variation. crown copyright (c) 2012 published by elsevier gmbh. all rights reserved. |
| 英文摘要 | nam is an important domestication gene and valuable to enhance grain protein contents (gpcs) of modern wheat cultivars. in the present study, 12 nam-g1 genes in triticum timopheevii zhuk. (aagg, 2n = 4x = 28) were cloned. these genes had the same length of 1546 bp including two introns and three exons, and encoded a polypeptide of 407 amino acid residues which contained a n-terminal nac domain with five sub-domains, and a c-terminal transcriptional activation region (tar). they were highly similar to the previously published functional nam-b1 gene dq871219 from t. turgidum ssp. dicoccoides korn. (aabb, 2n = 4x = 28) in both the nucleotide and protein sequence's, with a very high identity of 99.5%. the differences among the 12 nam-g1 genes resulted from 17 snps including 14 transitions and 3 transversions. they had outstandingly different expression levels in qrt-pcr. and, their relative expression quantities were significantly positively correlated with gpc of the accessions. in addition, the difference in amino acid sequences of the nam-g1 genes may also affect the gpc variation. crown copyright (c) 2012 published by elsevier gmbh. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | plant sciences |
| 研究领域[WOS] | plant sciences |
| 关键词[WOS] | nac transcription factors ; content locus ; wheat ; arabidopsis ; family ; domain ; domestication ; superfamily ; senescence ; hordeum |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000315310000012 |
| 公开日期 | 2014-05-09 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/3960]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Sichuan Agr Univ, Triticeae Res Inst, Wenjiang 611130, Peoples R China 2.Sichuan Agr Univ, Minist Educ, Key Lab Crop Genet Resources & Improvement, Yaan, Peoples R China 3.Chinese Acad Sci, NW Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China 4.Sichuan Agr Univ, Maize Res Inst, Wenjiang 611130, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Xi-Gui,Wu, Bi-Hua,Liu, Deng-Cai,et al. Variation and their relationship of NAM-G1 gene and grain protein content in Triticum timopheevii Zhuk[J]. journal of plant physiology,2013,170(3):330-337. |
| APA | Hu, Xi-Gui,Wu, Bi-Hua,Liu, Deng-Cai,Wei, Yu-Ming,Gao, Shi-Bin,&Zhen, You-Liang.(2013).Variation and their relationship of NAM-G1 gene and grain protein content in Triticum timopheevii Zhuk.journal of plant physiology,170(3),330-337. |
| MLA | Hu, Xi-Gui,et al."Variation and their relationship of NAM-G1 gene and grain protein content in Triticum timopheevii Zhuk".journal of plant physiology 170.3(2013):330-337. |
入库方式： OAI收割
来源：西北高原生物研究所
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