Preparative Isolation and Purification of Three Stilbene Glycosides from the Tibetan Medicinal Plant Rheum tanguticum Maxim. Ex Balf. by High-speed Counter-current Chromatography
文献类型：期刊论文
| 作者 | Zhao, Xiao-Hui1,2; Han, Fa1; Li, Yu-Lin1; Yue, Hui-Lan1 |
| 刊名 | phytochemical analysis
 |
| 出版日期 | 2013 |
| 卷号 | 24期号:2页码:171-175 |
| 关键词 | Preparative separation Cis-rhapontin Trans-desoxyrhaponticin Trans-rhapontin Rheum tanguticum Maxim. ex Balf. |
| ISSN号 | 0958-0344 |
| 通讯作者 | zhao, xh (reprint author), chinese acad sci, key lab adaptat & evolut plateau biota, northwest inst plateau biol, xining 810001, peoples r china. |
| 中文摘要 | introduction - stilbene glycosides are the primary constituents of rheum tanguticum maxim. ex balf., to which different bioactivities has been attributed, including: anti-hiv, anti-oxidant, anti-tumour, anti-malarial, and anti-allergy activity. however, effective methods for the isolation and purification of stilbene glycosides, such as trans-rhapontin, cis-rhapontin and trans-desoxyrhaponticin, from this herb are not currently available. objective - to develop an efficient method for the preparative isolation and purification of three stilbene glycosides from rheum tanguticum maxim. ex balf. via high-speed counter-current chromatography (hsccc). methods - a solvent system composed of chloroform: n-butanol: methanol: water (4:1:3:2, v/v/v/v) was developed for the separation. the upper phase was used as the stationary phase, and the lower phase was used as the mobile phase. the flow rate was 1.8 ml/min. the apparatus was controlled at 800 rpm and 25 degrees c, and the effluent was monitored at 280 nm. chemical constituents were analysed by high-performance liquid chromatography (hplc), and their structures were identified by h-1- and c-13-nmr. results - under the optimised conditions, 25.5 mg trans-rhapontin, 16.0 mg cis-rhapontin and 20.5 mg trans-desoxyrhaponticin were separated from 80 mg crude sample; the isolates had purities of 99.6, 97.2 and 99.2%, respectively. conclusion - a simple and efficient hsccc method has been optimised for the preparative separation of stilbene glycosides from rheum tanguticum maxim. ex balf. copyright (c) 2012 john wiley & sons, ltd. |
| 英文摘要 | introduction - stilbene glycosides are the primary constituents of rheum tanguticum maxim. ex balf., to which different bioactivities has been attributed, including: anti-hiv, anti-oxidant, anti-tumour, anti-malarial, and anti-allergy activity. however, effective methods for the isolation and purification of stilbene glycosides, such as trans-rhapontin, cis-rhapontin and trans-desoxyrhaponticin, from this herb are not currently available. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; plant sciences ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; plant sciences ; chemistry |
| 关键词[WOS] | polygonum-multiflorum ; parthenocissus-tricuspidata ; inhibitory constituents ; rhubarb ; undulatum ; requirements ; glucoside ; rhizoma ; alpha ; korea |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000318002600011 |
| 公开日期 | 2014-05-09 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/3975]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810001, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Xiao-Hui,Han, Fa,Li, Yu-Lin,et al. Preparative Isolation and Purification of Three Stilbene Glycosides from the Tibetan Medicinal Plant Rheum tanguticum Maxim. Ex Balf. by High-speed Counter-current Chromatography[J]. phytochemical analysis,2013,24(2):171-175. |
| APA | Zhao, Xiao-Hui,Han, Fa,Li, Yu-Lin,&Yue, Hui-Lan.(2013).Preparative Isolation and Purification of Three Stilbene Glycosides from the Tibetan Medicinal Plant Rheum tanguticum Maxim. Ex Balf. by High-speed Counter-current Chromatography.phytochemical analysis,24(2),171-175. |
| MLA | Zhao, Xiao-Hui,et al."Preparative Isolation and Purification of Three Stilbene Glycosides from the Tibetan Medicinal Plant Rheum tanguticum Maxim. Ex Balf. by High-speed Counter-current Chromatography".phytochemical analysis 24.2(2013):171-175. |
入库方式： OAI收割
来源：西北高原生物研究所
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