连续自由流电泳微重力下分离模式蛋白质的研究
文献类型：期刊论文
| 作者 | 丁士健 ; 刘韬 ; 宋金芳 ; 吴高德 ; 邵晓霞 ; 夏其昌 ; 吴汉基 ; 蒋远大 ; 张志远 ; 刘文喜 ; 冯学章 ; 马玉立 ; 李钦 |
| 刊名 | 空间科学学报
 |
| 出版日期 | 2004 |
| 卷号 | 24期号:3页码:233-240 |
| 关键词 | 连续自由流电泳 微重力 分离模式 蛋白质 血红蛋白 细胞色素C 空间实验 |
| ISSN号 | 0254-6124 |
| 其他题名 | FREE FLOW ELECTROPHORESIS SEPARATING MODEL PROTEINS UNDER THE MICROGRAVITY |
| 通讯作者 | 北京8701信箱 |
| 中文摘要 | 以连续自由流电泳的区带电泳模式分离血红蛋白和细胞色素C，比较了不同缓冲液甘油浓度下的分离效果，确定了最终装载在神舟4号飞船上A3—2型电泳仪的电泳分离条件，通过地面和空间实验，初步探讨了微重力对连续自由流电泳分离的影响，并对电泳的分离产物做了多种检测． |
| 英文摘要 | Separation of two colored model proteins, cytochrome Cand hemoglobin, were conducted in the specially self-designed free flow electrophoresis device A3-2 using zone electrophoresis mode under 1 g-and fig-conditions. The results demonstrated that the higher resolution of free flow electrophoresis acquired under the micrograv-ity is obtained. Both onboard LBD detection and measurement of the separation by the spectrometer demonstrated that the distance between the zone maxima of the two proteins were increased and the dispersion of hemoglobin decreased. The exper-invents also included: (1) The effect of the glycerol concentration on the separation results;(2) Post-flight analysis of the contents of the fractions. The separation results of 10%,7%, 4%, 2% and 0% glycerol concentration in the electrophoresis buffer are compared and no significant effect of the glycerol on the separation results are found.So the glycerol and its disadvantages for the clean and storage of the device were excluded finally.Gel electrophoresis and mass spectrometry analysis of the contents of the fractions confirmed that two proteins have been effectively separated. |
| 学科主题 | 空间技术 |
| 收录类别 | CSCD |
| 资助信息 | 国家载人航天工程项目 |
| 语种 | 中文 |
| CSCD记录号 | CSCD:1622760 |
| 源URL | [http://ir.cssar.ac.cn/handle/122/621]  |
| 专题 | 国家空间科学中心_空间技术部 |
| 推荐引用方式 GB/T 7714 | 丁士健,刘韬,宋金芳,等. 连续自由流电泳微重力下分离模式蛋白质的研究[J]. 空间科学学报,2004,24(3):233-240. |
| APA | 丁士健.,刘韬.,宋金芳.,吴高德.,邵晓霞.,...&李钦.(2004).连续自由流电泳微重力下分离模式蛋白质的研究.空间科学学报,24(3),233-240. |
| MLA | 丁士健,et al."连续自由流电泳微重力下分离模式蛋白质的研究".空间科学学报 24.3(2004):233-240. |
入库方式： OAI收割
来源：国家空间科学中心
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