中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 昆明植物研究所
  3. 中国科学院昆明植物研究所
  4. 植物化学与西部植物资源持续利用国家重点实验室
Cordycepol C Induces Caspase-Independent Apoptosis in Human Hepatocellular Carcinoma HepG2 Cells

文献类型：期刊论文

作者Sun, Yi-sheng1,2; Lv, Long-xian1; Zhao, Zhao1; He, Xian1; You, Lu3; Liu, Ji-kai4; Li, Yong-quan1
刊名BIOLOGICAL & PHARMACEUTICAL BULLETIN
出版日期2014-04-01
卷号37期号:4页码:608-617
关键词cordycepol C apoptosis bax protein apoptosis-inducing factor endonuclease G
ISSN号0918-6158
通讯作者Liu, JK (reprint author), Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650204, Peoples R China. ; jkliu@mail.kib.ac.cn ; lyq@zju.edu.cn
英文摘要Cordycepol C, a novel sesquiterpene isolated from the cultured mycelia of Cordyceps ophioglossoides, contains a hydroperoxy group and is cytotoxic to HepG2 cells. So far, no sesquiterpenes have been found in the genus Cordyceps and it would be interesting to investigate the antitumor efficacy as well as the mechanism of action of this unusual sesquiterpene. In this study, we showed that cordycepol C induced apoptosis of the HepG2 cells without affecting the normal liver cell line L-02. Cordycepol C caused poly(ADP-ribose) polymerase-1 (PARP-1) cleavage and triggered the loss of mitochondrial membrane potential (Delta psi(m)) in HepG2 cells in a time- and dose-dependent manner, resulting in the nuclear translocation of apoptosis-inducing factor (AIF) and endonuclease G (Endo G). We also found that cordycepol C induced the expression of Bax protein, followed by its translocation from the cytosol to mitochondria in both wild type and p53 knockdown HepG2 cells. However, cordycepol C could not cause cleavages of procaspase-3, -8, and -9. Caspase activities were not increased and Z-VAD-fmk, a caspase inhibitor, could not prevent the apoptosis induced by cordycepol C. These findings indicate that cordycepol C induces caspase-independent apoptosis in HepG2 cells through a p53-independent and Bax-mediated mitochondrial pathway, leading to the nuclear translocation of AIF and Endo G. Our study provides the molecular mechanism by which cordycepol C induces apoptosis in hepatocellular carcinoma cells and indicates the potential use of cordycepol C as an antitumor agent.
学科主题Pharmacology & Pharmacy
类目[WOS]Pharmacology & Pharmacy
研究领域[WOS]Pharmacology & Pharmacy
关键词[WOS]ENDONUCLEASE-G ; PROGRAMMED NECROSIS ; OPHIOGLOSSOIDES L2 ; CANCER CELLS ; DEATH ; MITOCHONDRIA ; ACTIVATION ; RELEASE ; CULTURE ; BAX
收录类别SCI
资助信息National High Technology Research and Development Program of China [2007AA021506]; Natural Science Foundation of Zhejiang Province, China [R207609]
语种英语
WOS记录号WOS:000333577800014
公开日期2014-05-16
源URL[http://ir.kib.ac.cn/handle/151853/18043]  
专题昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室
作者单位1.Zhejiang Univ, Coll Life Sci, Hangzhou 310058, Zhejiang, Peoples R China
2.Zhejiang Prov Ctr Dis Control & Prevent, Hangzhou 310051, Zhejiang, Peoples R China
3.Huangyan Tradit Chinese Med Hosp, Dept Orthoped, Taizhou 318000, Peoples R China
4.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650204, Peoples R China
推荐引用方式
GB/T 7714		 Sun, Yi-sheng,Lv, Long-xian,Zhao, Zhao,et al. Cordycepol C Induces Caspase-Independent Apoptosis in Human Hepatocellular Carcinoma HepG2 Cells[J]. BIOLOGICAL & PHARMACEUTICAL BULLETIN,2014,37(4):608-617.
APA Sun, Yi-sheng.,Lv, Long-xian.,Zhao, Zhao.,He, Xian.,You, Lu.,...&Li, Yong-quan.(2014).Cordycepol C Induces Caspase-Independent Apoptosis in Human Hepatocellular Carcinoma HepG2 Cells.BIOLOGICAL & PHARMACEUTICAL BULLETIN,37(4),608-617.
MLA Sun, Yi-sheng,et al."Cordycepol C Induces Caspase-Independent Apoptosis in Human Hepatocellular Carcinoma HepG2 Cells".BIOLOGICAL & PHARMACEUTICAL BULLETIN 37.4(2014):608-617.

入库方式： OAI收割

来源：昆明植物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。