Biosensor Based on Malic Dehydrogenase Immobilized in a CdS-Graphene-Chitosan Nanocomposite for Root-Exuded Malic Acid Determination
文献类型：期刊论文
| ; | |
| 作者 | Wu Yanyou; Niu Huiyang; Wang Kun; Liu Qian; Li Henan; Dong Xiaoya; Zhao Kuan; Zhu Yongli |
| 刊名 | Sensor Letters
 ; Sensor Letters
|
| 出版日期 | 2013 ; 2013 |
| 卷号 | 11期号:2页码:436-441 |
| 其他题名 | Wu Yanyou; Niu Huiyang; Wang Kun; Liu Qian; Li Henan; Dong Xiaoya; Zhao Kuan; Zhu Yongli |
| 英文摘要 | A new measurement method for root-exuded malic acid based on CdS-graphene-chitosan (CdS-G-CHIT) nanocomposite was reported. The preparation of CdS-G-CHIT was performed by dispersing CdS-G nanocomposite in chitosan solution. The amperometric biosensor was constructed by coating CdS-graphene-chitosan (CdS-G-CHIT) nanocomposite on glassy carbon electrode (GCE), and then adsorbing malic dehydrogenase (MDH). The pH in a pyrophosphate buffer solution, the concentration of oxidized nicotinamide adenine dinucleotide (NAD(+)), and the potential applied in the measurement system on the current response of the fabricated biosensor was investigated. Under optimum conditions, the biosensor showed an acceptable linear response from 1.8 mu M to 120 mu M, with a detection limit of 0.6 mu M. Moreover, the biosensor exhibited high specificity, good stability, and reproducibility. This approach was suitable for the direct measurement of malic acid in root exudates. |
| 学科主题 | 环境地球化学 ; 环境地球化学 |
| 收录类别 | SCI |
| 语种 | 英语 ; 英语 |
| 公开日期 | 2014-06-04 ; 2014-06-04 |
| 源URL | [http://119.78.100.189:8080/handle/352002/5059]  |
| 专题 | 地球化学研究所_环境地球化学国家重点实验室_环境地球化学国家重点实验室_期刊论文 |
| 推荐引用方式 GB/T 7714 | Wu Yanyou,Niu Huiyang,Wang Kun,et al. Biosensor Based on Malic Dehydrogenase Immobilized in a CdS-Graphene-Chitosan Nanocomposite for Root-Exuded Malic Acid Determination, Biosensor Based on Malic Dehydrogenase Immobilized in a CdS-Graphene-Chitosan Nanocomposite for Root-Exuded Malic Acid Determination[J]. Sensor Letters, Sensor Letters,2013, 2013,11, 11(2):436-441, 436-441. |
| APA | Wu Yanyou.,Niu Huiyang.,Wang Kun.,Liu Qian.,Li Henan.,...&Zhu Yongli.(2013).Biosensor Based on Malic Dehydrogenase Immobilized in a CdS-Graphene-Chitosan Nanocomposite for Root-Exuded Malic Acid Determination.Sensor Letters,11(2),436-441. |
| MLA | Wu Yanyou,et al."Biosensor Based on Malic Dehydrogenase Immobilized in a CdS-Graphene-Chitosan Nanocomposite for Root-Exuded Malic Acid Determination".Sensor Letters 11.2(2013):436-441. |
入库方式： OAI收割
来源：地球化学研究所
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