Effects of nitrogen application rate and a nitrification inhibitor dicyandiamide on methanotroph abundance and methane uptake in a grazed pasture soil
文献类型:期刊论文
作者 | Dai, Yu ; Di, Hong J. ; Cameron, Keith C. ; He, Ji-Zheng |
刊名 | ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
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出版日期 | 2013 |
卷号 | 20期号:12页码:8680-8689 |
通讯作者 | He, JZ |
中文摘要 | Methane-oxidizing bacteria (methanotrophs) in the soil are a unique group of methylotrophic bacteria that utilize methane (CH4) as their sole source of carbon and energy which limit the flux of methane to the atmosphere from soils and consume atmospheric methane. A field experiment was conducted to determine the effect of nitrogen application rates and the nitrification inhibitor dicyandiamide (DCD) on the abundance of methanotrophs and on methane flux in a grazed pasture soil. Nitrogen (N) was applied at four different rates, with urea applied at 50 and 100 kg N ha(-1) and animal urine at 300 and 600 kg N ha(-1). DCD was applied at 10 kg ha(-1). The results showed that both the DNA and selected mRNA copy numbers of the methanotroph pmoA gene were not affected by the application of urea, urine or DCD. The methanotroph DNA and mRNA pmoA gene copy numbers were low in this soil, below 7.13 x 10(3) g(-1) soil and 3.75 x 10(3) mu g(-1) RNA, respectively. Daily CH4 flux varied slightly among different treatments during the experimental period, ranging from -12.89 g CH4 ha(-1) day(-1) to -0.83 g CH4 ha(-1) day(-1), but no significant treatment effect was found. This study suggests that the application of urea fertilizer, animal urine returns and the use of the nitrification inhibitor DCD do not significantly affect soil methanotroph abundance or daily CH4 fluxes in grazed grassland soils. |
收录类别 | SCI |
WOS记录号 | WOS:000327498600038 |
公开日期 | 2014-06-04 |
源URL | [http://ir.rcees.ac.cn/handle/311016/7259] ![]() |
专题 | 生态环境研究中心_城市与区域生态国家重点实验室 |
推荐引用方式 GB/T 7714 | Dai, Yu,Di, Hong J.,Cameron, Keith C.,et al. Effects of nitrogen application rate and a nitrification inhibitor dicyandiamide on methanotroph abundance and methane uptake in a grazed pasture soil[J]. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH,2013,20(12):8680-8689. |
APA | Dai, Yu,Di, Hong J.,Cameron, Keith C.,&He, Ji-Zheng.(2013).Effects of nitrogen application rate and a nitrification inhibitor dicyandiamide on methanotroph abundance and methane uptake in a grazed pasture soil.ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH,20(12),8680-8689. |
MLA | Dai, Yu,et al."Effects of nitrogen application rate and a nitrification inhibitor dicyandiamide on methanotroph abundance and methane uptake in a grazed pasture soil".ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH 20.12(2013):8680-8689. |
入库方式: OAI收割
来源:生态环境研究中心
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