Mechanism of gold nanoparticle induced simultaneously increased PCR efficiency and specificity
文献类型：期刊论文
| 作者 | Lin Yan1; Li Jia2; Yao Jing2; Liang Yong2,3; Zhang Jie1; Zhou QunFang4; Jiang GuiBin4 |
| 刊名 | CHINESE SCIENCE BULLETIN
 |
| 出版日期 | 2013-12-01 |
| 卷号 | 58期号:36页码:4593-4601 |
| 关键词 | gold nanoparticles PCR efficiency PCR specificity thermal conductivity single-stranded DNA binding protein |
| 通讯作者 | Liang, Y |
| 中文摘要 | Gold nanoparticles (AuNPs) have been reported to induce faster heat transfer of polymerase chain reaction (PCR) solution to enhance PCR efficiency. AuNPs can also increase the specificity of PCR by functioning analogously to single-stranded DNA binding protein (SSB). However, the simple structure of AuNPs makes it difficult to determine how AuNPs affect PCR efficiency and specificity. This study aimed to elucidate the effect of AuNPs on PCR efficiency by altering the denaturation times and annealing temperatures. In addition, comparative study of the effect of AuNPs on PCR inhibition caused by two competitive primers allowed investigation of the mechanisms of AuNP-enhanced PCR accuracy. Finally, heat-treated salmon sperm DNA was used to evaluate whether AuNPs could eliminate the inhibitory PCR effect caused by DNA impurities. This study demonstrated that enhanced thermal conductivity by AuNPs was the main mechanism for increased PCR efficiency and specificity. AuNPs promoted efficient double-stranded DNA template unwinding and dissociation between mismatched primers, DNA fragments and template, and enhanced PCR efficiency and specificity simultaneously. Thus, this significant finding suggests the future use of AuNP-assisted PCR in different fields, especially in rapid clinical diagnosis and screening by PCR. |
| 英文摘要 | Gold nanoparticles (AuNPs) have been reported to induce faster heat transfer of polymerase chain reaction (PCR) solution to enhance PCR efficiency. AuNPs can also increase the specificity of PCR by functioning analogously to single-stranded DNA binding protein (SSB). However, the simple structure of AuNPs makes it difficult to determine how AuNPs affect PCR efficiency and specificity. This study aimed to elucidate the effect of AuNPs on PCR efficiency by altering the denaturation times and annealing temperatures. In addition, comparative study of the effect of AuNPs on PCR inhibition caused by two competitive primers allowed investigation of the mechanisms of AuNP-enhanced PCR accuracy. Finally, heat-treated salmon sperm DNA was used to evaluate whether AuNPs could eliminate the inhibitory PCR effect caused by DNA impurities. This study demonstrated that enhanced thermal conductivity by AuNPs was the main mechanism for increased PCR efficiency and specificity. AuNPs promoted efficient double-stranded DNA template unwinding and dissociation between mismatched primers, DNA fragments and template, and enhanced PCR efficiency and specificity simultaneously. Thus, this significant finding suggests the future use of AuNP-assisted PCR in different fields, especially in rapid clinical diagnosis and screening by PCR. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | SINGLE-STRANDED-DNA ; BINDING-PROTEIN ; REAL-TIME ; FIDELITY ; SIZE ; AMPLIFICATION ; POLYMERASE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000327482400011 |
| 公开日期 | 2014-06-06 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/7450]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Huazhong Agr Univ, Minist Agr, Key Lab Subtrop Agr & Environm, Wuhan 430070, Peoples R China 2.Jianghan Univ, Sch Med, Wuhan 430056, Peoples R China 3.Jianghan Univ, Minist Educ, Key Lab Optoelect Chem Mat & Devices, Wuhan 430056, Peoples R China 4.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lin Yan,Li Jia,Yao Jing,et al. Mechanism of gold nanoparticle induced simultaneously increased PCR efficiency and specificity[J]. CHINESE SCIENCE BULLETIN,2013,58(36):4593-4601. |
| APA | Lin Yan.,Li Jia.,Yao Jing.,Liang Yong.,Zhang Jie.,...&Jiang GuiBin.(2013).Mechanism of gold nanoparticle induced simultaneously increased PCR efficiency and specificity.CHINESE SCIENCE BULLETIN,58(36),4593-4601. |
| MLA | Lin Yan,et al."Mechanism of gold nanoparticle induced simultaneously increased PCR efficiency and specificity".CHINESE SCIENCE BULLETIN 58.36(2013):4593-4601. |
入库方式： OAI收割
来源：生态环境研究中心
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