Detection and characterization of Bonamia ostreae in Ostrea edulis imported to China
文献类型：期刊论文
| 作者 | Feng, Chunyan ; Lin, Xiangmei ; Wang, Fei ; Zhang, Yongning ; Lv, Jizhou ; Wang, Caixia ; Deng, Junhua ; Mei, Lin ; Wu, Shaoqiang ; Li, Huailin |
| 刊名 | DISEASES OF AQUATIC ORGANISMS
 |
| 出版日期 | 2013 |
| 卷号 | 106期号:1页码:85-91 |
| 通讯作者 | Wu, SQ |
| 中文摘要 | The protozoan parasite Bonamia ostreae is a destructive pathogen of flat oysters and has been reported to be widespread in Europe and North America. The biological characteristics of this unicellular parasite are still not fully understood. In this study, 104 Ostrea edulis imported from the USA to the Guangdong province of China for consumption were examined for Bonamia infection. PCR assay, combined with restriction fragment length polymorphism, sequencing and BLAST analysis, showed that B. ostreae DNA could be detected in 1 of the 104 oyster samples. Light microscopy revealed Bonamia-like organisms in the oyster. PCR assay and fluorescent in situ hybridization showed that B. ostreae organisms were present and retained their integrity after 4 wk in culture. Acridine orange-ethidium bromide staining indicated that the B. ostreae were still alive. In conclusion, B. ostreae was present in oysters imported to China. More importantly, the parasite was able to survive for at least 4 wk of in vitro culture at 4 degrees C, which further implied a long-term transmission risk of B. ostreae. Considering the wide culture beds of Crassostrea ariakensis and C. gigas in China, and that C. ariakensis and C. gigas are susceptible hosts or reservoirs of B. ostreae, our study highlights the potential risk of introducing B. ostreae by importing O. edulis from a Bonamia endemic area. |
| 收录类别 | SCI |
| WOS记录号 | WOS:000324887300010 |
| 公开日期 | 2014-06-06 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/7473]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Feng, Chunyan,Lin, Xiangmei,Wang, Fei,et al. Detection and characterization of Bonamia ostreae in Ostrea edulis imported to China[J]. DISEASES OF AQUATIC ORGANISMS,2013,106(1):85-91. |
| APA | Feng, Chunyan.,Lin, Xiangmei.,Wang, Fei.,Zhang, Yongning.,Lv, Jizhou.,...&Li, Huailin.(2013).Detection and characterization of Bonamia ostreae in Ostrea edulis imported to China.DISEASES OF AQUATIC ORGANISMS,106(1),85-91. |
| MLA | Feng, Chunyan,et al."Detection and characterization of Bonamia ostreae in Ostrea edulis imported to China".DISEASES OF AQUATIC ORGANISMS 106.1(2013):85-91. |
入库方式： OAI收割
来源：生态环境研究中心
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