An Ammonium Bicarbonate-Enhanced Stable Isotope Dilution UHPLC-MS/MS Method for Sensitive and Accurate Quantification of Acrolein-DNA Adducts in Human Leukocytes
文献类型：期刊论文
| 作者 | Yin, Ruichuan ; Liu, Shengquan ; Zhao, Chao ; Lu, Meiling ; Tang, Moon-shong ; Wang, Hailin |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2013 |
| 卷号 | 85期号:6页码:3190-3197 |
| 通讯作者 | Wang, HL |
| 中文摘要 | Acrolein (Acr), a ubiquitous environmental pollutant, can react directly with genomic DNA to form mutagenic adducts without undergoing metabolic activation. To sensitively and accurately quantify Acr-DNA adducts (including structural isomers and stereoisomers) in human leukocytes, we developed an enhanced stable isotope dilution ultrahigh performance liquid chromatography (UHPLC)-tandem mass spectrometry (MS/MS) method using ammonium bicarbonate (NH4HCO3), which is thermally unstable and degrades readily to carbon dioxide and ammonia in heated gas phase. Interestingly, ammonium bicarbonate (as an additive to the mobile phase) not only improves the protonation of AcrdG adducts but also suppresses the formation of MS signal-deteriorating metal-AcrdG complexes during electrospray ionization, leading to the enhancement of their MS detection by 2.3-8.7 times. In contrast, routinely used ammonium salts (ammonium acetate and ammonium formate) and formic acid do not show similar enhancement. The developed method is potentially useful for enhancing ESI-MS detection of other modified 2'-deoxyribonudeosides that have difficulty in protonation and may form excess metal complexes during electrospray ionization. The limits of detection (LODs, S/N = 3) are estimated to be about 40-80 amol. By the use of the developed method, we found that the Acr adducts of three nucleotides (dG, dA, and dC) can be detected in human leukocytes. In addition to the known yAcrdG, alpha-AcrdA is also identified as an Acr-adduct of high abundance (2.5-20 adducts per10(8) nts). |
| 收录类别 | SCI |
| WOS记录号 | WOS:000316520500022 |
| 公开日期 | 2014-06-06 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/7515]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Yin, Ruichuan,Liu, Shengquan,Zhao, Chao,et al. An Ammonium Bicarbonate-Enhanced Stable Isotope Dilution UHPLC-MS/MS Method for Sensitive and Accurate Quantification of Acrolein-DNA Adducts in Human Leukocytes[J]. ANALYTICAL CHEMISTRY,2013,85(6):3190-3197. |
| APA | Yin, Ruichuan,Liu, Shengquan,Zhao, Chao,Lu, Meiling,Tang, Moon-shong,&Wang, Hailin.(2013).An Ammonium Bicarbonate-Enhanced Stable Isotope Dilution UHPLC-MS/MS Method for Sensitive and Accurate Quantification of Acrolein-DNA Adducts in Human Leukocytes.ANALYTICAL CHEMISTRY,85(6),3190-3197. |
| MLA | Yin, Ruichuan,et al."An Ammonium Bicarbonate-Enhanced Stable Isotope Dilution UHPLC-MS/MS Method for Sensitive and Accurate Quantification of Acrolein-DNA Adducts in Human Leukocytes".ANALYTICAL CHEMISTRY 85.6(2013):3190-3197. |
入库方式： OAI收割
来源：生态环境研究中心
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