Polyvinyl Pyrrolidone Promotes DNA Cleavage by a ROS-Independent and Depurination Mechanism
文献类型：期刊论文
| 作者 | Song, Maoyong ; Zeng, Luzhe ; Hong, Xianjun ; Meng, Zihui ; Yin, Junfa ; Wang, Hailin ; Liang, Yong ; Jiang, Guibin |
| 刊名 | ENVIRONMENTAL SCIENCE & TECHNOLOGY
 |
| 出版日期 | 2013 |
| 卷号 | 47期号:6页码:2886-2891 |
| 通讯作者 | Wang, HL |
| 中文摘要 | Polyvinyl pyrrolidone polymer (PVP) has been widely applied in biological and medical fields. A few in vitro studies indicated that PVP might cause toxicity. However, the underlying mechanism is poorly understood. In this work, we found that PVP directly induced strand breakages of various DNA molecules, implicating a cleavage activity. Moreover, reactive oxygen species (ROS) scavenging analysis shows that DNA cleavage activity of PVP is not related to ROS-induced oxidation. As revealed by gel electrophoresis and liquid chromatography/mass spectrometry analysis, the major cleavage products of DNA were identified as two purine bases, guanine and adenine, suggesting that PVPs have a novel depurination activity. The selective depurination and DNA cleavage activity of PVPs were further confirmed by studying the interaction of PVP with four nucleosides and four well-designed oligodeoxynucleotides probes containing specific nucleotides. This study may provide insights into PVP-DNA interactions and resultant genotoxicity and may also open a new way for DNA study. |
| 收录类别 | SCI |
| WOS记录号 | WOS:000316594000058 |
| 公开日期 | 2014-06-06 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/7516]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Song, Maoyong,Zeng, Luzhe,Hong, Xianjun,et al. Polyvinyl Pyrrolidone Promotes DNA Cleavage by a ROS-Independent and Depurination Mechanism[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2013,47(6):2886-2891. |
| APA | Song, Maoyong.,Zeng, Luzhe.,Hong, Xianjun.,Meng, Zihui.,Yin, Junfa.,...&Jiang, Guibin.(2013).Polyvinyl Pyrrolidone Promotes DNA Cleavage by a ROS-Independent and Depurination Mechanism.ENVIRONMENTAL SCIENCE & TECHNOLOGY,47(6),2886-2891. |
| MLA | Song, Maoyong,et al."Polyvinyl Pyrrolidone Promotes DNA Cleavage by a ROS-Independent and Depurination Mechanism".ENVIRONMENTAL SCIENCE & TECHNOLOGY 47.6(2013):2886-2891. |
入库方式： OAI收割
来源：生态环境研究中心
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