Establishment of quantitative PCR methods for the quantification of geosmin-producing potential and Anabaena sp in freshwater systems
文献类型：期刊论文
| 作者 | Su, Ming ; Gaget, Virginie ; Giglio, Steven ; Burch, Michael ; An, Wei ; Yang, Min |
| 刊名 | WATER RESEARCH
 |
| 出版日期 | 2013 |
| 卷号 | 47期号:10页码:3444-3454 |
| 通讯作者 | Yang, M |
| 中文摘要 | Geosmin has often been associated with off-flavor problems in drinking water with Anabaena sp. as the major producer. Rapid on-site detection of geosmin-producers as well as geosmin is important for a timely management response to potential off-flavor events. In this study, quantitative polymerase chain reaction (qPCR) methods were developed to detect the levels of Anabaena sp. and geosmin, respectively, by designing two PCR primer sets to quantify the rpoC(1) gene (ARG) and geosmin synthase one (GSG) in Anabaena sp. in freshwater systems. The ARG density determined by qPCR assay is highly related to microscopic cell count (r(2) = 0.726, p < 0.001), and the limit of detection (LOD) and limit of quantification (LOQ) of the qPCR method were 0.02 pg and 0.2 pg of DNA, respectively. At the same time, the relationship between geosmin concentrations measured by gas chromatography-mass spectrometry (GC-MS) and GSG copies was also established (r(2) = 0.742, p < 0.001) with similar LOD and LOQ values. Using the two qPCR protocols, we succeeded in measuring different levels of ARG and GSG copies in different freshwater systems with high incidence environmental substrata and diverse ecological conditions, showing that the methods developed could be applied for environmental monitoring. Moreover, comparing to the microscopic count and GC MS analytical methods, the qPCR methods can reduce the time-to-results from several days to a few hours and require considerably less traditional algal identification and taxonomic expertise. (C) 2013 Elsevier Ltd. All rights reserved. |
| 收录类别 | SCI |
| WOS记录号 | WOS:000319644800017 |
| 公开日期 | 2014-06-06 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/7412]  |
| 专题 | 生态环境研究中心_环境水质学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Su, Ming,Gaget, Virginie,Giglio, Steven,et al. Establishment of quantitative PCR methods for the quantification of geosmin-producing potential and Anabaena sp in freshwater systems[J]. WATER RESEARCH,2013,47(10):3444-3454. |
| APA | Su, Ming,Gaget, Virginie,Giglio, Steven,Burch, Michael,An, Wei,&Yang, Min.(2013).Establishment of quantitative PCR methods for the quantification of geosmin-producing potential and Anabaena sp in freshwater systems.WATER RESEARCH,47(10),3444-3454. |
| MLA | Su, Ming,et al."Establishment of quantitative PCR methods for the quantification of geosmin-producing potential and Anabaena sp in freshwater systems".WATER RESEARCH 47.10(2013):3444-3454. |
入库方式： OAI收割
来源：生态环境研究中心
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