Gold nanoparticle-assisted primer walking for closing the human chromosomal gap
文献类型：期刊论文
| 作者 | Li, Haikuo ; Shi, Baochen ; Li, Xueling ; Nyegaard, Steffen ; Lu, Ming ; Lu, Junhong ; Cao, Xueyan ; Huang, Qing（黄庆） ; Fan, Chunhai（樊春海） ; Chen, Runsheng ; Hu, Jun（胡钧） |
| 刊名 | ANALYTICAL METHODS
 |
| 出版日期 | 2013 |
| 卷号 | 5期号:18页码:CONCATENATE(Sheet1!I139,-Sheet1!J139) |
| ISSN号 | 1759-9660 |
| 英文摘要 | The finished sequence of the human genome still contains 260 euchromatic gaps. All the PCR-based genome walking techniques used to close gaps have common limitations, such as low efficiency and low specificity. We herein describe a strategy to solve this problem by employing gold nanoparticles (AuNPs) to improve the efficiency in primer walking amplification. We used this strategy to close a gap in human chromosome 5 containing a DNA stretch composed of the 12SAT repeat. The obtained gap sequence is highly conserved among several mammalian genomes. The demonstrated AuNP-assisted primer walking strategy is capable of effectively improving the specificity of PCR amplification and enriching the yield of target DNA fragments; it offers a new avenue for closing gaps left by current sequencing methods. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000323518200029 |
| 公开日期 | 2014-06-13 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/13618]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Li, Haikuo,Shi, Baochen,Li, Xueling,et al. Gold nanoparticle-assisted primer walking for closing the human chromosomal gap[J]. ANALYTICAL METHODS,2013,5(18):CONCATENATE(Sheet1!I139,-Sheet1!J139). |
| APA | Li, Haikuo.,Shi, Baochen.,Li, Xueling.,Nyegaard, Steffen.,Lu, Ming.,...&Hu, Jun.(2013).Gold nanoparticle-assisted primer walking for closing the human chromosomal gap.ANALYTICAL METHODS,5(18),CONCATENATE(Sheet1!I139,-Sheet1!J139). |
| MLA | Li, Haikuo,et al."Gold nanoparticle-assisted primer walking for closing the human chromosomal gap".ANALYTICAL METHODS 5.18(2013):CONCATENATE(Sheet1!I139,-Sheet1!J139). |
入库方式： OAI收割
来源：上海应用物理研究所
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