A Graphene Oxide-Based Fluorescent Biosensor for the Analysis of Peptide-Receptor Interactions and Imaging in Somatostatin Receptor Subtype 2 Overexpressed Tumor Cells
文献类型：期刊论文
| 作者 | Feng Bianying ; Guo Linjie ; Wang Lihua（王丽华） ; Li Fan ; Lu Jianxin ; Gao Jimin ; Fan Chunhai（樊春海） ; Huang Qing（黄庆） |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2013 |
| 卷号 | 85期号:16页码:CONCATENATE(Sheet1!I8,-Sheet1!J8) |
| ISSN号 | 0003-2700 |
| 英文摘要 | Analysis of peptide-receptor interactions provides insights for understanding functions of proteins in cells. In this work, we report the development of a fluorescent biosensor for the analysis of peptide-receptor interactions using graphene oxide (GO) and fluorescein isothiocyanate (FITC)-labeled octreotide (FOC). Octreotide is a synthesized cyclic peptide with somatostatin-like bioactivity that has been clinically employed. FOC exhibits high adsorption affinity for GO, and its binding results in efficient fluorescence quenching of FITC. Interestingly, the specific binding of the antibody anti-octreotide (AOC) with FOC competitively releases FOC from the GO surface, leading to the recovery of fluorescence. By using this GO-based fluorescent platform, we can detect AOC with a low detection limit of 2 ng/mL. As a step further, we employ this GO-FOC biosensor to image somatostatin receptor subtype 2 overexpressed AR42J tumor cells, which demonstrates high promise for molecular imaging in cancer diagnosis. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000323471800018 |
| 公开日期 | 2014-06-13 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/13652]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Feng Bianying,Guo Linjie,Wang Lihua,et al. A Graphene Oxide-Based Fluorescent Biosensor for the Analysis of Peptide-Receptor Interactions and Imaging in Somatostatin Receptor Subtype 2 Overexpressed Tumor Cells[J]. ANALYTICAL CHEMISTRY,2013,85(16):CONCATENATE(Sheet1!I8,-Sheet1!J8). |
| APA | Feng Bianying.,Guo Linjie.,Wang Lihua.,Li Fan.,Lu Jianxin.,...&Huang Qing.(2013).A Graphene Oxide-Based Fluorescent Biosensor for the Analysis of Peptide-Receptor Interactions and Imaging in Somatostatin Receptor Subtype 2 Overexpressed Tumor Cells.ANALYTICAL CHEMISTRY,85(16),CONCATENATE(Sheet1!I8,-Sheet1!J8). |
| MLA | Feng Bianying,et al."A Graphene Oxide-Based Fluorescent Biosensor for the Analysis of Peptide-Receptor Interactions and Imaging in Somatostatin Receptor Subtype 2 Overexpressed Tumor Cells".ANALYTICAL CHEMISTRY 85.16(2013):CONCATENATE(Sheet1!I8,-Sheet1!J8). |
入库方式： OAI收割
来源：上海应用物理研究所
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