A Highly Sensitive Amperometric Immunosensor for Clenbuterol Detection in Livestock Urine
文献类型：期刊论文
| 作者 | Li, Shengxi ; Zhang, Jiong ; Deng, Wangping ; Shen, Xizhong ; Song, Shiping（宋世平） ; Fan, Chunhai(樊春海） |
| 刊名 | ELECTROANALYSIS
 |
| 出版日期 | 2013 |
| 卷号 | 25期号:4页码:CONCATENATE(Sheet1!I10,-Sheet1!J10) |
| ISSN号 | 1040-0397 |
| 英文摘要 | We report a highly sensitive competitive-type assay for multiplexing electrochemical detection of clenbuterol in pig urine using a 16-sensor array. In this design, the clenbuterol was first conjugated with bovine serum albumin (BSA), and then the conjugates were immobilized on the electrode surface to compete with the free clenbuterol in the sample for specific antibody. Under the optimal conditions, the reproducibility of the clenbuterol electrochemical immunosensor was evaluated to be 3.4% (coefficient of variation, CV) and the limit of detection was estimated to be 1.3pg/mL. The very low detection limit was probably derived from the higher efficiency of the competitive immunoreaction caused by appropriate quantities of the clenbuterol immobilized on the 16-sensor array and the suitable amount of anti-clenbuterol antibody in the assay system. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000317677400009 |
| 公开日期 | 2014-06-13 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/13653]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Li, Shengxi,Zhang, Jiong,Deng, Wangping,et al. A Highly Sensitive Amperometric Immunosensor for Clenbuterol Detection in Livestock Urine[J]. ELECTROANALYSIS,2013,25(4):CONCATENATE(Sheet1!I10,-Sheet1!J10). |
| APA | Li, Shengxi,Zhang, Jiong,Deng, Wangping,Shen, Xizhong,Song, Shiping,&Fan, Chunhai.(2013).A Highly Sensitive Amperometric Immunosensor for Clenbuterol Detection in Livestock Urine.ELECTROANALYSIS,25(4),CONCATENATE(Sheet1!I10,-Sheet1!J10). |
| MLA | Li, Shengxi,et al."A Highly Sensitive Amperometric Immunosensor for Clenbuterol Detection in Livestock Urine".ELECTROANALYSIS 25.4(2013):CONCATENATE(Sheet1!I10,-Sheet1!J10). |
入库方式： OAI收割
来源：上海应用物理研究所
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