A Novel Electromagnetic Apparatus for Rapid Multiplex Single Molecule Force Spectroscopy
文献类型:期刊论文
作者 | Shen, Yi ; Czajkowsky, Daniel M. ; Li, Xiaowei ; Sun, Jielin ; Hu, Jun(胡钧) ; Shao, Zhifeng |
刊名 | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY |
出版日期 | 2013 |
卷号 | 13期号:2页码:CONCATENATE(Sheet1!I14,-Sheet1!J14) |
ISSN号 | 1533-4880 |
英文摘要 | Single-molecule force spectroscopy has revolutionized our ability to probe the details of molecular structures and interactions, but the numbers of individual measurements required for achieving a statistically reliable result can sometimes prove daunting. To overcome this problem, a number of instruments have recently been developed that are capable of monitoring the behavior of tens of individual biomolecules simultaneously. In this work, we have constructed a novel electromagnetic apparatus for multiplex single molecule force measurements utilizing magnetic microspheres. In this system, the magnetic field is generated with an electron-lens of an electron microscope mated with a high voltage flash light circuit to rapidly attain a stable magnetic field. We show that this instrument can generate a uniform magnetic force of up to similar to 20 pN within 5 ms, over a region spanning 1 mm. The successful application of this apparatus to the force-dependent extension of dsDNA fully validates this approach. Furthermore, the lens-like design of the pole piece is fully compatible with optical imaging, thus allowing for the integration of single molecule fluorescence capabilities that should make this system a particularly powerful apparatus for multi-dimensional characterization of fast processes within interacting single molecules. |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000318254500105 |
公开日期 | 2014-06-13 |
源URL | [http://ir.sinap.ac.cn/handle/331007/13657] |
专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
推荐引用方式 GB/T 7714 | Shen, Yi,Czajkowsky, Daniel M.,Li, Xiaowei,et al. A Novel Electromagnetic Apparatus for Rapid Multiplex Single Molecule Force Spectroscopy[J]. JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY,2013,13(2):CONCATENATE(Sheet1!I14,-Sheet1!J14). |
APA | Shen, Yi,Czajkowsky, Daniel M.,Li, Xiaowei,Sun, Jielin,Hu, Jun,&Shao, Zhifeng.(2013).A Novel Electromagnetic Apparatus for Rapid Multiplex Single Molecule Force Spectroscopy.JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY,13(2),CONCATENATE(Sheet1!I14,-Sheet1!J14). |
MLA | Shen, Yi,et al."A Novel Electromagnetic Apparatus for Rapid Multiplex Single Molecule Force Spectroscopy".JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY 13.2(2013):CONCATENATE(Sheet1!I14,-Sheet1!J14). |
入库方式: OAI收割
来源:上海应用物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。