A surface-initiated enzymatic polymerization strategy for electrochemical DNA sensors
文献类型：期刊论文
| 作者 | Wan, Ying ; Xu, Hui ; Su, Yan ; Zhu, Xinhua ; Song, Shiping（宋世平） ; Fan, Chunhai（樊春海） |
| 刊名 | BIOSENSORS & BIOELECTRONICS
 |
| 出版日期 | 2013 |
| 卷号 | 41页码:CONCATENATE(Sheet1!I22,-Sheet1!J22) |
| ISSN号 | 0956-5663 |
| 英文摘要 | In this work, we report a novel strategy of electrochemical DNA (E-DNA) sensor based on surface initiated enzymatic polymerization (SIEP). This DNA sensor employs a capture DNA probe labeled with thiol at its 3' terminal to be immobilized at gold electrode via gold-thiol chemistry. Oligo (ethylene glycol) -terminated thiols (SH-OEGs) are then used to prepare an oligonucleotide-incorporated nonfouling surface (ONS). After the sequence-specific recognition of target DNA, terminal deoxynucleotidyl transferase (TdT) is employed to catalyze the sequential addition of deoxynucleotides (dNTPs) at the 3'-OH group of target DNA without template. During the TdT-mediated extension reaction, by using biotinlated 2'-deoxyadenosine 5'-triphosphate (biotin-dATP), biotin labels are incorporated into the SIEP-generated long single-stranded DNA (ssDNA). Specific binding of avidin-horseradish peroxidase (Av-HRP) to the biotin label leads to enzyme turnover-based signal transduction. By using this new strategy, we demonstrated the high picomolar sensitivity and a broad detection range of six orders of magnitude. (C) 2012 Elsevier B.V. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000314191300078 |
| 公开日期 | 2014-06-13 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/13662]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Wan, Ying,Xu, Hui,Su, Yan,et al. A surface-initiated enzymatic polymerization strategy for electrochemical DNA sensors[J]. BIOSENSORS & BIOELECTRONICS,2013,41:CONCATENATE(Sheet1!I22,-Sheet1!J22). |
| APA | Wan, Ying,Xu, Hui,Su, Yan,Zhu, Xinhua,Song, Shiping,&Fan, Chunhai.(2013).A surface-initiated enzymatic polymerization strategy for electrochemical DNA sensors.BIOSENSORS & BIOELECTRONICS,41,CONCATENATE(Sheet1!I22,-Sheet1!J22). |
| MLA | Wan, Ying,et al."A surface-initiated enzymatic polymerization strategy for electrochemical DNA sensors".BIOSENSORS & BIOELECTRONICS 41(2013):CONCATENATE(Sheet1!I22,-Sheet1!J22). |
入库方式： OAI收割
来源：上海应用物理研究所
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