Development of a microchip-pulsed electrochemical method for rapid determination of L-DOPA and tyrosine in Mucuna pruriens
文献类型：期刊论文
| 作者 | Li, Xinchun ; Chen, Zuanguang ; Yang, Fan ; Pan, Jianbin ; Li, Yinbao |
| 刊名 | JOURNAL OF SEPARATION SCIENCE
 |
| 出版日期 | 2013 |
| 卷号 | 36期号:41892页码:CONCATENATE(Sheet1!I80,-Sheet1!J80) |
| ISSN号 | 1615-9306 |
| 英文摘要 | L-3,4-dihydroxyphenylalanine (L-DOPA) is a well-recognized therapeutic compound to Parkinson's disease. Tyrosine is a precursor for the biosynthesis of L-DOPA, both of which are widely found in traditional medicinal material, Mucuna pruriens. In this paper, we described a validated novel analytical method based on microchip capillary electrophoresis with pulsed electrochemical detection for the simultaneous measurement of L-DOPA and tyrosine in M. pruriens. This protocol adopted end-channel amperometric detection using platinum disk electrode on a homemade glass/polydimethylsiloxane electrophoresis microchip. The background buffer consisted of 10 mM borate (pH 9.5) and 0.02 mM cetyltrimethylammonium bromide, which can produce an effective resolution for the two analytes. In the optimal condition, sufficient electrophoretic separation and sensitive detection for the target analytes can be realized within 60 s. Both tyrosine and L-DOPA yielded linear response in the concentration range of 5.0-400 M (R2 > 0.99), and the LOD were 0.79 and 1.1 M, respectively. The accuracy and precision of the established method were favorable. The present method shows several merits such as facile apparatus, high speed, low cost and minimal pollution, and provides a means for the pharmacologically active ingredients assay in M. pruriens. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000319866800013 |
| 公开日期 | 2014-06-13 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/13710]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Li, Xinchun,Chen, Zuanguang,Yang, Fan,et al. Development of a microchip-pulsed electrochemical method for rapid determination of L-DOPA and tyrosine in Mucuna pruriens[J]. JOURNAL OF SEPARATION SCIENCE,2013,36(41892):CONCATENATE(Sheet1!I80,-Sheet1!J80). |
| APA | Li, Xinchun,Chen, Zuanguang,Yang, Fan,Pan, Jianbin,&Li, Yinbao.(2013).Development of a microchip-pulsed electrochemical method for rapid determination of L-DOPA and tyrosine in Mucuna pruriens.JOURNAL OF SEPARATION SCIENCE,36(41892),CONCATENATE(Sheet1!I80,-Sheet1!J80). |
| MLA | Li, Xinchun,et al."Development of a microchip-pulsed electrochemical method for rapid determination of L-DOPA and tyrosine in Mucuna pruriens".JOURNAL OF SEPARATION SCIENCE 36.41892(2013):CONCATENATE(Sheet1!I80,-Sheet1!J80). |
入库方式： OAI收割
来源：上海应用物理研究所
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