Diagnosis of schistosomiasis japonica with interfacial co-assembly-based multi-channel electrochemical immunosensor arrays
文献类型：期刊论文
| 作者 | Deng, Wangping ; Xu, Bin ; Hu, Haiyan ; Li, Jianyong ; Hu, Wei ; Song, Shiping（宋世平） ; Feng, Zheng ; Fan, Chunhai（樊春海） |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2013 |
| 卷号 | 3 |
| ISSN号 | 2045-2322 |
| 英文摘要 | Schistosomiasis control remains to be an important and challenging task in the world. However, lack of quick, simple, sensitive and specific sero-diagnostic test is still a hurdle in the control practice. The commonly employed enzyme-linked immuno-sorbent assay (ELISA) relies on the native soluble egg antigen (SEA) that is limited in supply. Here we developed an electrochemical immunosensor array (ECISA) assay with an interfacial co-assembly strategy. A recombinant Schistosoma japonicum (Sj) calcium-binding protein (SjE16) was used as a principal antigen, while the SEA as a minor, co-assembling agent, with a ratio of 8 : 1 (SjE16: SEA, Sj16EA), which was co-immobilized on a disposable 16-channel screen-printed carbon electrode array. A portable electrochemical detector was employed to detect antibodies in serum samples. The sensitivity of ECISA reached 100% with minimal cross-reactions. Therefore, we have demonstrated that this rapid, sensitive and specific ECISA technique has the potential to perform large-scale on-site screening of Sj infection. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000318471000003 |
| 公开日期 | 2014-06-13 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/13713]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Deng, Wangping,Xu, Bin,Hu, Haiyan,et al. Diagnosis of schistosomiasis japonica with interfacial co-assembly-based multi-channel electrochemical immunosensor arrays[J]. SCIENTIFIC REPORTS,2013,3. |
| APA | Deng, Wangping.,Xu, Bin.,Hu, Haiyan.,Li, Jianyong.,Hu, Wei.,...&Fan, Chunhai.(2013).Diagnosis of schistosomiasis japonica with interfacial co-assembly-based multi-channel electrochemical immunosensor arrays.SCIENTIFIC REPORTS,3. |
| MLA | Deng, Wangping,et al."Diagnosis of schistosomiasis japonica with interfacial co-assembly-based multi-channel electrochemical immunosensor arrays".SCIENTIFIC REPORTS 3(2013). |
入库方式： OAI收割
来源：上海应用物理研究所
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