Differential pulsed amperometry coupled to microchip capillary electrophoresis
文献类型：期刊论文
| 作者 | Li, Xinchun ; Chen, Zuanguang ; Pan, Jianbin ; Yang, Fan ; Li, Yinbao ; Yao, Meicun |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2013 |
| 卷号 | 1291页码:CONCATENATE(Sheet1!I85,-Sheet1!J85) |
| ISSN号 | 0021-9673 |
| 英文摘要 | In this work, we describe a novel electrochemical detection method, differential pulsed amperometry (DPA) on microchip capillary electrophoresis (MCE). In a pulse period, a sequential two-step sampling is executed at two different potentials (E-1 and E-2). Differential current signal of the duplex sampling events is recorded that functions as time domain. The performance of this detection scheme was evaluated by separating and detecting three model analytes including tyramine (Tym), tryptophan (Trp), and p-aminobenzoic acid (PABA). Multiple parameters that would affect electrochemical response and peak shape, such as sampling potential, sampling time, and electrode cleaning time, were investigated. This pulse technique exhibits better sensitivity over constant potential amperometry (CPA), nearly equal to triple pulsed amperometry (TPA). More importantly, DPA can generate more stable baseline than TPA, primarily due to the background subtraction through the two-step sampling, which is beneficial to further improve analytical sensitivity. In the optimal condition, the limits of detection for Tym, Trp and PABA, were down to 0.27 mu M, 0.32 mu M and 1.1 mu M, respectively. DPA detection opens up a new avenue for microchip electrochemistry, and can be virtually extended to other fluid analysis techniques. (C) 2013 Elsevier B.V. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000318745400021 |
| 公开日期 | 2014-06-13 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/13715]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Li, Xinchun,Chen, Zuanguang,Pan, Jianbin,et al. Differential pulsed amperometry coupled to microchip capillary electrophoresis[J]. JOURNAL OF CHROMATOGRAPHY A,2013,1291:CONCATENATE(Sheet1!I85,-Sheet1!J85). |
| APA | Li, Xinchun,Chen, Zuanguang,Pan, Jianbin,Yang, Fan,Li, Yinbao,&Yao, Meicun.(2013).Differential pulsed amperometry coupled to microchip capillary electrophoresis.JOURNAL OF CHROMATOGRAPHY A,1291,CONCATENATE(Sheet1!I85,-Sheet1!J85). |
| MLA | Li, Xinchun,et al."Differential pulsed amperometry coupled to microchip capillary electrophoresis".JOURNAL OF CHROMATOGRAPHY A 1291(2013):CONCATENATE(Sheet1!I85,-Sheet1!J85). |
入库方式： OAI收割
来源：上海应用物理研究所
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