Graphene-based nanoprobes and a prototype optical biosensing platform
文献类型：期刊论文
| 作者 | Xu, Hui ; Wang, Dongfang ; He, Shijiang ; Li, Jiang ; Feng, Bianying ; Ma, Peijun ; Xu, Pingping ; Gao, Shanmin ; Zhang, Shengxiao ; Liu, Quanwen ; Lu, Jianxin ; Song, Shiping（宋世平） ; Fan, Chunhai（樊春海） |
| 刊名 | BIOSENSORS & BIOELECTRONICS
 |
| 出版日期 | 2013 |
| 卷号 | 50页码:CONCATENATE(Sheet1!I141,-Sheet1!J141) |
| ISSN号 | 0956-5663 |
| 英文摘要 | Biochemical and biomedical applications of graphene are critically dependent on the interaction between biomolecules and the nanomaterial. In this work, we developed a graphene-based signal-amplification nanoprobe by combining anti-immunoglobulin G (anti-IgG) and horseradish peroxidase (HRP) with graphene oxide (GO). The structure and function of HRP in the nano-interface of GO were firstly investigated, which demonstrated that the enzyme retained 78% of its native activity and 77% of its native a-helix content HRP and anti-IgG were then co-adsorbed onto GO to form bifunctional nanoprobes. The nanoprobes provide both improved binding ability and signal-amplification ability. Comparing with conventional bioconjugates such as enzyme-linked antibody, co-adsorption could avoid chemical conjugation between biomolecules, keeping their bioactivity well. As an example for their application, the nanoprobes were used to obtain amplified signals in a sandwich-type immunoassay for cancer marker, instead of conventional colorimetric conjugates. This approach provided a detection limit of 10 pg/mL alpha-fetoprotein (AFP), which was much more sensitive than conventional enzyme-linked immunosorbent assay (ELISA) methods. The easily fabricated GO-based nanoprobes have the potential to become universal probes for molecular diagnostics. (C) 2013 Elsevier B.V. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000325188200040 |
| 公开日期 | 2014-06-13 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/13762]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Xu, Hui,Wang, Dongfang,He, Shijiang,et al. Graphene-based nanoprobes and a prototype optical biosensing platform[J]. BIOSENSORS & BIOELECTRONICS,2013,50:CONCATENATE(Sheet1!I141,-Sheet1!J141). |
| APA | Xu, Hui.,Wang, Dongfang.,He, Shijiang.,Li, Jiang.,Feng, Bianying.,...&Fan, Chunhai.(2013).Graphene-based nanoprobes and a prototype optical biosensing platform.BIOSENSORS & BIOELECTRONICS,50,CONCATENATE(Sheet1!I141,-Sheet1!J141). |
| MLA | Xu, Hui,et al."Graphene-based nanoprobes and a prototype optical biosensing platform".BIOSENSORS & BIOELECTRONICS 50(2013):CONCATENATE(Sheet1!I141,-Sheet1!J141). |
入库方式： OAI收割
来源：上海应用物理研究所
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