Highly sensitive recognition of Pb2+ using Pb2+ triggered exonuclease aided DNA recycling
文献类型：期刊论文
| 作者 | Xu, Hui ; Xu, Pingping ; Gao, Shanmin ; Zhang, Shengxiao ; Zhao, Xingchun ; Fan, Chunhai（樊春海） ; Zuo, Xiaolei（左小磊） |
| 刊名 | BIOSENSORS & BIOELECTRONICS
 |
| 出版日期 | 2013 |
| 卷号 | 47页码:CONCATENATE(Sheet1!I151,-Sheet1!J151) |
| ISSN号 | 0956-5663 |
| 英文摘要 | Here, we have demonstrated an ultra-high sensitive detection platform with the detection limit of 5 pM for an environmental toxin-Pb2+. We designed a Pb2+ triggered exonuclease aided DNA recycling system to improve the detection sensitivity. In our system, a Pb2+ dependent 8-17 DNAzyme and its substrate were used to form hybridization duplex. In the presence of Pb2+, the substrate was cleaved and disassociated from the duplex. Then, the released 8-17 DNAzyme was used as a target of the exonuclease aided DNA recycling system which can amplify the fluorescence signal by recycling the 8-17 DNAzyme continuously. Then, the sensitive Pb2+ detection are accomplished and the detection limit of Pb2+ was down to 5 pM which is about 1000 times lower than the traditional detection method based on the 8-17 DNAzyme. (C) 2013 Elsevier B.V. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000320481900079 |
| 公开日期 | 2014-06-13 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/13768]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Xu, Hui,Xu, Pingping,Gao, Shanmin,et al. Highly sensitive recognition of Pb2+ using Pb2+ triggered exonuclease aided DNA recycling[J]. BIOSENSORS & BIOELECTRONICS,2013,47:CONCATENATE(Sheet1!I151,-Sheet1!J151). |
| APA | Xu, Hui.,Xu, Pingping.,Gao, Shanmin.,Zhang, Shengxiao.,Zhao, Xingchun.,...&Zuo, Xiaolei.(2013).Highly sensitive recognition of Pb2+ using Pb2+ triggered exonuclease aided DNA recycling.BIOSENSORS & BIOELECTRONICS,47,CONCATENATE(Sheet1!I151,-Sheet1!J151). |
| MLA | Xu, Hui,et al."Highly sensitive recognition of Pb2+ using Pb2+ triggered exonuclease aided DNA recycling".BIOSENSORS & BIOELECTRONICS 47(2013):CONCATENATE(Sheet1!I151,-Sheet1!J151). |
入库方式： OAI收割
来源：上海应用物理研究所
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