Imaging cells and sub-cellular structures with ultrahigh resolution full-field X-ray microscopy
文献类型：期刊论文
| 作者 | Chien, C. C. ; Tseng, P. Y. ; Chen, H. H. ; Hua, T. E. ; Chen, S. T. ; Chen, Y. Y. ; Leng, W. H. ; Wang, C. H. ; Hwu, Y. ; Yin, G. C. ; Liang, K. S. ; Chen, F. R. ; Chu, Y. S. ; Yeh, H. I. ; Yang, Y. C. ; Yang, C. S. ; Zhang, G. L. ; Je, J. H. ; Margaritondo, G. |
| 刊名 | BIOTECHNOLOGY ADVANCES
 |
| 出版日期 | 2013 |
| 卷号 | 31期号:3页码:CONCATENATE(Sheet1!I159,-Sheet1!J159) |
| ISSN号 | 0734-9750 |
| 英文摘要 | Our experimental results demonstrate that full-field hard-X-ray microscopy is finally able to investigate the internal structure of cells in tissues. This result was made possible by three main factors: the use of a coherent (synchrotron) source of X-rays, the exploitation of contrast mechanisms based on the real part of the refractive index and the magnification provided by high-resolution Fresnel zone-plate objectives. We specifically obtained high-quality microradiographs of human and mouse cells with 29 nm Rayleigh spatial resolution and verified that tomographic reconstruction could be implemented with a final resolution level suitable for subcellular features. We also demonstrated that a phase retrieval method based on a wave propagation algorithm could yield good subcellular images starting from a series of defocused microradiographs. The concluding discussion compares cellular and subcellular hard-X-ray microradiology with other techniques and evaluates its potential impact on biomedical research. (C) 2012 Elsevier Inc. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000317441900004 |
| 公开日期 | 2014-06-13 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/13775]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Chien, C. C.,Tseng, P. Y.,Chen, H. H.,et al. Imaging cells and sub-cellular structures with ultrahigh resolution full-field X-ray microscopy[J]. BIOTECHNOLOGY ADVANCES,2013,31(3):CONCATENATE(Sheet1!I159,-Sheet1!J159). |
| APA | Chien, C. C..,Tseng, P. Y..,Chen, H. H..,Hua, T. E..,Chen, S. T..,...&Margaritondo, G..(2013).Imaging cells and sub-cellular structures with ultrahigh resolution full-field X-ray microscopy.BIOTECHNOLOGY ADVANCES,31(3),CONCATENATE(Sheet1!I159,-Sheet1!J159). |
| MLA | Chien, C. C.,et al."Imaging cells and sub-cellular structures with ultrahigh resolution full-field X-ray microscopy".BIOTECHNOLOGY ADVANCES 31.3(2013):CONCATENATE(Sheet1!I159,-Sheet1!J159). |
入库方式： OAI收割
来源：上海应用物理研究所
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