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Lab in a Tube: Ultrasensitive Detection of MicroRNAs at the Single-Cell Level and in Breast Cancer Patients Using Quadratic Isothermal Amplification
文献类型：期刊论文
| 作者 | Duan, Ruixue ; Zuo, Xiaolei ; Wang, Shutao ; Quan, Xiyun ; Chen, Dongliang ; Chen, Zhifei ; Jiang, Lei ; Fan, Chunhai ; Xia, Fan |
| 刊名 | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
 |
| 出版日期 | 2013 |
| 卷号 | 135期号:12页码:CONCATENATE(Sheet1!I188,-Sheet1!J188) |
| ISSN号 | 0002-7863 |
| 英文摘要 | Through rational design of a functional molecular probe with high sequence specificity that takes advantage of sensitive isothermal amplification with simple operation, we developed a one-pot hairpin-mediated quadratic enzymatic amplification strategy for microRNA (miRNA) detection. Our method exhibits ultrahigh sensitivity toward miR-21 with detection limits of 10 fM at 37 degrees C and 1 aM at 4 degrees C, which corresponds to nine strands of miR-21 in a 15 mu L sample, and it is capable of distinguishing among miRNA family members. More importantly, the proposed approach is also sensitive and selective when applied to crude extractions from MCF-7 and PC3 cell lines and even patient tissues from intraductal carcinoma and invasive ductal carcinoma of the breast. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000317032000008 |
| 公开日期 | 2014-06-13 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/13800]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Duan, Ruixue,Zuo, Xiaolei,Wang, Shutao,et al. Lab in a Tube: Ultrasensitive Detection of MicroRNAs at the Single-Cell Level and in Breast Cancer Patients Using Quadratic Isothermal Amplification[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2013,135(12):CONCATENATE(Sheet1!I188,-Sheet1!J188). |
| APA | Duan, Ruixue.,Zuo, Xiaolei.,Wang, Shutao.,Quan, Xiyun.,Chen, Dongliang.,...&Xia, Fan.(2013).Lab in a Tube: Ultrasensitive Detection of MicroRNAs at the Single-Cell Level and in Breast Cancer Patients Using Quadratic Isothermal Amplification.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,135(12),CONCATENATE(Sheet1!I188,-Sheet1!J188). |
| MLA | Duan, Ruixue,et al."Lab in a Tube: Ultrasensitive Detection of MicroRNAs at the Single-Cell Level and in Breast Cancer Patients Using Quadratic Isothermal Amplification".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 135.12(2013):CONCATENATE(Sheet1!I188,-Sheet1!J188). |
入库方式： OAI收割
来源：上海应用物理研究所
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