Probing tethered targets of a single biomolecular complex with atomic force microscopy
文献类型：期刊论文
| 作者 | Wu, Na ; Wang, Qi ; Zhou, Xingfei ; Jia, Si Si ; Fan, Youjie ; Hu, Jun ; Li, Bin |
| 刊名 | JOURNAL OF MOLECULAR RECOGNITION
 |
| 出版日期 | 2013 |
| 卷号 | 26期号:12页码:CONCATENATE(Sheet1!I264,-Sheet1!J264) |
| ISSN号 | 0952-3499; 1099-1352 |
| 英文摘要 | DNA origami shows tremendous promise as templates for the assembly of nano-components and detection of molecular recognition events. So far, the method of choice for evaluating these structures has been atomic force microscopy (AFM), a powerful tool for imaging nanoscale objects. In most cases, tethered targets on DNA origami have proven to be highly effective samples for investigation. Still, while maximal assembly of the nanostructures might benefit from the greatest flexibility in the tether, AFM imaging requires a sufficient stability of the adsorbed components. The balance between the tether flexibility and sample stability is a major, poorly understood, concern in such studies. Here, we investigated the dependence of the tethering length on molecular capture events monitored by AFM. In our experiments, single biotin molecules were attached to DNA origami templates with various linker lengths of thymidine nucleotides, and their interaction with streptavidin was observed with AFM. Our results show that the streptavidin-biotin complexes are easily detected with short tethered lengths, and that their morphological features clearly change with the tethering length. We identify the functionally useful tether lengths for these investigations, which are also expected to prove useful in the construction and further application of DNA origami in bio-nanotechnology studies. Copyright (c) 2013 John Wiley & Sons, Ltd. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000327375400013 |
| 公开日期 | 2014-06-13 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/13867]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Wu, Na,Wang, Qi,Zhou, Xingfei,et al. Probing tethered targets of a single biomolecular complex with atomic force microscopy[J]. JOURNAL OF MOLECULAR RECOGNITION,2013,26(12):CONCATENATE(Sheet1!I264,-Sheet1!J264). |
| APA | Wu, Na.,Wang, Qi.,Zhou, Xingfei.,Jia, Si Si.,Fan, Youjie.,...&Li, Bin.(2013).Probing tethered targets of a single biomolecular complex with atomic force microscopy.JOURNAL OF MOLECULAR RECOGNITION,26(12),CONCATENATE(Sheet1!I264,-Sheet1!J264). |
| MLA | Wu, Na,et al."Probing tethered targets of a single biomolecular complex with atomic force microscopy".JOURNAL OF MOLECULAR RECOGNITION 26.12(2013):CONCATENATE(Sheet1!I264,-Sheet1!J264). |
入库方式： OAI收割
来源：上海应用物理研究所
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