Real Time in Vitro Regulation of DNA Methylation Using a 5-Fluorouracil Conjugated DNA-Based Stimuli-Responsive Platform
文献类型：期刊论文
| 作者 | Mao, Xiuhai ; Wei, Ming ; Zhu, Chengfeng ; Lu, Jianxin ; Gao, Jimin ; Simon, Anna J. ; Shi, Jiye ; Huang, Qing ; Fan, Chunhai |
| 刊名 | ACS APPLIED MATERIALS & INTERFACES
 |
| 出版日期 | 2013 |
| 卷号 | 5期号:7页码:CONCATENATE(Sheet1!I279,-Sheet1!J279) |
| ISSN号 | 1944-8244 |
| 英文摘要 | DNA methylation, catalyzed by methylases, plays a critical role in many biological processes, and many methylases have been regarded as promising targets for antimicrobial drugs. In this work, we report a stimulus responsive, self-regulating anticancer drug release platform, comprising a multifunctional DNA that upon methylation by methyltransferase (MTase) releases 5-fluorouracil (5-Fu) and in turn inhibits subsequent expression of MTase. The multifunctional DNA with anticancer drug are first methylated by DNA adenine methylation (DAM) methyltransferase (MTase) and then cut by the methylation-sensitive restriction endonuclease Dpn I. Removal of duplex from the functional DNA by the methylation/cleavage process will release the anticancer drug, resulting in inhibition of the activity of DAM in turn. Consequently, the enzyme activity of DAM MTase can be self-regulated. Furthermore, we found that the inhibition efficiency of 5-Fu significantly increase as it is functionalized with DNA. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000317549100043 |
| 公开日期 | 2014-06-13 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/13882]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Mao, Xiuhai,Wei, Ming,Zhu, Chengfeng,et al. Real Time in Vitro Regulation of DNA Methylation Using a 5-Fluorouracil Conjugated DNA-Based Stimuli-Responsive Platform[J]. ACS APPLIED MATERIALS & INTERFACES,2013,5(7):CONCATENATE(Sheet1!I279,-Sheet1!J279). |
| APA | Mao, Xiuhai.,Wei, Ming.,Zhu, Chengfeng.,Lu, Jianxin.,Gao, Jimin.,...&Fan, Chunhai.(2013).Real Time in Vitro Regulation of DNA Methylation Using a 5-Fluorouracil Conjugated DNA-Based Stimuli-Responsive Platform.ACS APPLIED MATERIALS & INTERFACES,5(7),CONCATENATE(Sheet1!I279,-Sheet1!J279). |
| MLA | Mao, Xiuhai,et al."Real Time in Vitro Regulation of DNA Methylation Using a 5-Fluorouracil Conjugated DNA-Based Stimuli-Responsive Platform".ACS APPLIED MATERIALS & INTERFACES 5.7(2013):CONCATENATE(Sheet1!I279,-Sheet1!J279). |
入库方式： OAI收割
来源：上海应用物理研究所
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