Salts drive controllable multilayered upright assembly of amyloid-like peptides at mica/water interface
文献类型：期刊论文
| 作者 | Dai, Bin ; Kang, Seung-gu ; Tien Huynh ; Lei, Haozhi ; Castelli, Matteo ; Hu, Jun ; Zhang, Yi ; Zhou, Ruhong |
| 刊名 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
 |
| 出版日期 | 2013 |
| 卷号 | 110期号:21页码:CONCATENATE(Sheet1!I290,-Sheet1!J290) |
| ISSN号 | 0027-8424 |
| 英文摘要 | Surface-assisted self-assembly of amyloid-like peptides has received considerable interest in both amyloidosis research and nanotechnology in recent years. Despite extensive studies, some controlling factors, such as salts, are still not well understood, even though it is known that some salts can promote peptide self-assemblies through the so-called "salting-out" effect. However, they are usually noncontrollable, disordered, amorphous aggregates. Here, we show via a combined experimental and theoretical approach that a conserved consensus peptide NH2-VGGAVVAGV-CONH2 (GAV-9) (from representative amyloidogenic proteins) can self-assemble into highly ordered, multilayered nanofilaments, with surprising all-upright conformations, under high-salt concentrations. Our atomic force microscopy images also demonstrate that the vertical stacking of multiple layers is highly controllable by tuning the ionic strength, such as from 0 mM (monolayer) to 100 mM (mainly double layer), and to 250 mM MgCl2 (double, triple, quadruple, and quintuple layers). Our atomistic molecular dynamics simulations then reveal that these individual layers have very different internal nanostructures, with parallel beta-sheets in the first monolayer but antiparallel beta-sheets in the subsequent upper layers due to their different microenvironment. Further studies show that the growth of multilayered, all-upright nanostructures is a common phenomenon for GAV-9 at the mica/water interface, under a variety of salt types and a wide range of salt concentrations. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000320328700056 |
| 公开日期 | 2014-06-13 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/13892]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Dai, Bin,Kang, Seung-gu,Tien Huynh,et al. Salts drive controllable multilayered upright assembly of amyloid-like peptides at mica/water interface[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2013,110(21):CONCATENATE(Sheet1!I290,-Sheet1!J290). |
| APA | Dai, Bin.,Kang, Seung-gu.,Tien Huynh.,Lei, Haozhi.,Castelli, Matteo.,...&Zhou, Ruhong.(2013).Salts drive controllable multilayered upright assembly of amyloid-like peptides at mica/water interface.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,110(21),CONCATENATE(Sheet1!I290,-Sheet1!J290). |
| MLA | Dai, Bin,et al."Salts drive controllable multilayered upright assembly of amyloid-like peptides at mica/water interface".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 110.21(2013):CONCATENATE(Sheet1!I290,-Sheet1!J290). |
入库方式： OAI收割
来源：上海应用物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。