Single-nucleotide polymorphism genotyping using a novel multiplexed electrochemical biosensor with nonfouling surface
文献类型：期刊论文
| 作者 | Liu, Gang ; Lao, Ruojun ; Xu, Li ; Xu, Qin ; Li, Lanying ; Zhang, Min ; Song, Shiping ; Fan, Chunhai |
| 刊名 | BIOSENSORS & BIOELECTRONICS
 |
| 出版日期 | 2013 |
| 卷号 | 42页码:CONCATENATE(Sheet1!I303,-Sheet1!J303) |
| ISSN号 | 0956-5663 |
| 英文摘要 | A novel electrochemical DNA biosensor for single-nucleotide polymorphism (SNP) analysis was developed. In this work, an oligonucleotide-incorporated nonfouling surface (ONS) was constructed to resist nonspecific absorption. The biosensor was developed using a 16-electrode array for high-throughput SNP analysis. The proposed strategy was primarily based on specific oligonucleotide ligation. Fully matched target DNA templated the ligation between a capture probe assembled on gold electrodes and a tandem signal probe with a biotin moiety that could capture avidin-horseradish peroxidase and sequentially generate a catalysed amperometric signal. A pre-core mutation in the hepatitis B virus (HBV) genome at G1896A and two adjacent polymorphisms in the human CYP2C19 genome at C680T and G681A were analysed. Polymerase chain reaction (PCR) products were used as real-life samples and analysed. Our results showed that 10% of a single-mismatched mutant gene was clearly distinguished with a current signal 16 times higher than that of the blank sample, demonstrating the selectivity and practicability of the multiplexed electrochemical DNA biosensor. (C) 2012 Elsevier B.V. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000319951700085 |
| 公开日期 | 2014-06-13 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/13903]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Liu, Gang,Lao, Ruojun,Xu, Li,et al. Single-nucleotide polymorphism genotyping using a novel multiplexed electrochemical biosensor with nonfouling surface[J]. BIOSENSORS & BIOELECTRONICS,2013,42:CONCATENATE(Sheet1!I303,-Sheet1!J303). |
| APA | Liu, Gang.,Lao, Ruojun.,Xu, Li.,Xu, Qin.,Li, Lanying.,...&Fan, Chunhai.(2013).Single-nucleotide polymorphism genotyping using a novel multiplexed electrochemical biosensor with nonfouling surface.BIOSENSORS & BIOELECTRONICS,42,CONCATENATE(Sheet1!I303,-Sheet1!J303). |
| MLA | Liu, Gang,et al."Single-nucleotide polymorphism genotyping using a novel multiplexed electrochemical biosensor with nonfouling surface".BIOSENSORS & BIOELECTRONICS 42(2013):CONCATENATE(Sheet1!I303,-Sheet1!J303). |
入库方式： OAI收割
来源：上海应用物理研究所
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