Single-Step Rapid Assembly of DNA Origami Nanostructures for Addressable Nanoscale Bioreactors
文献类型：期刊论文
| 作者 | Fu, Yanming ; Zeng, Dongdong ; Chao, Jie ; Jin, Yanqiu ; Zhang, Zhao ; Liu, Huajie ; Li, Di ; Ma, Hongwei ; Huang, Qing ; Gothelf, Kurt V. ; Fan, Chunhai |
| 刊名 | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
 |
| 出版日期 | 2013 |
| 卷号 | 135期号:2页码:CONCATENATE(Sheet1!I304,-Sheet1!J304) |
| ISSN号 | 0002-7863 |
| 英文摘要 | Self-assembled DNA origami nanostructures have shown great promise for bottom-up construction of complex objects with nanoscale addressability. Here we show that DNA origami-based ID nanoribbons and nanotubes are one-pot assembled with controllable sizes and nanoscale addressability with high speed (within only 10-20 min), exhibiting extraordinarily high cooperativity that is often observed in assembly of natural molecular machines in cells (e.g. ribosome). By exploiting the high specificity of DNA-based self-assembly, we can precisely anchor proteins on these DNA origami nanostructures with sub-10 nm resolution and at the single-molecule level. We attach a pair of enzymes (horseradish peroxidase and glucose oxidase) at the inner side of DNA nanotubes and observe high coupling efficiency of enzyme cascade within this confined nanospace. Hence, DNA nanostructures with such unprecedented properties shed new light on the design of nanoscale bioreactors and nanomedicine and provide an artificial system for studying enzyme activities and cascade in highly organized and crowded cell-mimicking environments. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000313920800037 |
| 公开日期 | 2014-06-13 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/13904]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Fu, Yanming,Zeng, Dongdong,Chao, Jie,et al. Single-Step Rapid Assembly of DNA Origami Nanostructures for Addressable Nanoscale Bioreactors[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2013,135(2):CONCATENATE(Sheet1!I304,-Sheet1!J304). |
| APA | Fu, Yanming.,Zeng, Dongdong.,Chao, Jie.,Jin, Yanqiu.,Zhang, Zhao.,...&Fan, Chunhai.(2013).Single-Step Rapid Assembly of DNA Origami Nanostructures for Addressable Nanoscale Bioreactors.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,135(2),CONCATENATE(Sheet1!I304,-Sheet1!J304). |
| MLA | Fu, Yanming,et al."Single-Step Rapid Assembly of DNA Origami Nanostructures for Addressable Nanoscale Bioreactors".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 135.2(2013):CONCATENATE(Sheet1!I304,-Sheet1!J304). |
入库方式： OAI收割
来源：上海应用物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。