Adenanthin targets proteins involved in the regulation of disulphide bonds
文献类型：期刊论文
| 作者 | Muchowicz, Angelika1; Firczuk, Malgorzata1; Chlebowska, Justyna1; Nowis, Dominika1,2; Stachura, Joanna1; Barankiewicz, Joanna1; Trzeciecka, Anna1; Klossowski, Szymon3; Ostaszewski, Ryszard3; Zagozdzon, Radoslaw1 |
| 刊名 | BIOCHEMICAL PHARMACOLOGY
 |
| 出版日期 | 2014-05-15 |
| 卷号 | 89期号:2页码:210-216 |
| 关键词 | Adenanthin Cancer Peroxiredoxin Protein disulphide isomerase Thioredoxin |
| ISSN号 | 0006-2952 |
| 通讯作者 | Golab,J (reprint author),Med Univ Warsaw,Dept Immunol,Ctr Biostruct Res,1a Banacha Str,F Bldg,PL-02097 Warsaw,Poland. ; hdsun@mail.kib.ac.cn ; jakub.golab@wum.edu.pl |
| 英文摘要 | Adenanthin has been recently shown to inhibit the enzymatic activities of peroxiredoxins (Prdx) land II through its functional alpha,beta-unsaturated ketone group serving as a Michael acceptor. A similar group is found in SK053, a compound recently developed by our group to target the thioredoxin-thioredoxin reductase (Trx-TrxR) system. This work provides evidence that next to Prdx I and II adenanthin targets additional proteins including thioredoxin-thioredoxin reductase system as well as protein disulfide isomerase (PDI) that contain a characteristic structural motif, referred to as a thioredoxin fold. Adenanthin inhibits the activity of Trx-TR system and PDI in vitro in the insulin reduction assay and decreases the activity of Trx in cultured cells. Moreover, we identified Trx-1 as an adenanthin binding protein in cells incubated with biotinylated adenanthin as an affinity probe. The results of our studies indicate that adenanthin is a mechanism-selective, rather than an enzyme-specific inhibitor of enzymes containing readily accessible, nucleophilic cysteines. This observation might be of importance in considering potential therapeutic applications of adenanthin to include a range of diseases, where aberrant activity of Prdx, Trx-TrxR and PDI is involved in their pathogenesis. (C) 2014 Elsevier Inc. All rights reserved. |
| 学科主题 | Pharmacology & Pharmacy |
| 类目[WOS] | Pharmacology & Pharmacy |
| 研究领域[WOS] | Pharmacology & Pharmacy |
| 关键词[WOS] | THIOREDOXIN ; REDUCTION ; MECHANISM ; CANCER ; SYSTEM ; CELLS |
| 收录类别 | SCI |
| 资助信息 | Ministry of Science and Higher Education [NN405127640, IP2011 012671, IP2012 048172, 12011 038971]; Medical University of Warsaw [1M19/PM13D/12/12]; National Science Centre [2012/07/B/NZ7/04183]; National Natural Science Foundation of China [21322204]; European Commission [P7-REGPOT-2012-CT2012-316254-BASTION]; Polish L'Oreal-UNESCO Awards for Women in Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000335428800006 |
| 公开日期 | 2014-06-23 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/18118]  |
| 专题 | 昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室 |
| 作者单位 | 1.Med Univ Warsaw, Dept Immunol, Ctr Biostruct Res, PL-02097 Warsaw, Poland 2.Med Univ Warsaw, Dept Gen Transplant & Liver Surg, PL-02097 Warsaw, Poland 3.Polish Acad Sci, Inst Organ Chem, PL-01224 Warsaw, Poland 4.Chinese Acad Sci, Kunming Inst Bot, Kunming, Peoples R China 5.Polish Acad Sci, Inst Phys Chem, Dept 3, Warsaw, Poland |
| 推荐引用方式 GB/T 7714 | Muchowicz, Angelika,Firczuk, Malgorzata,Chlebowska, Justyna,et al. Adenanthin targets proteins involved in the regulation of disulphide bonds[J]. BIOCHEMICAL PHARMACOLOGY,2014,89(2):210-216. |
| APA | Muchowicz, Angelika.,Firczuk, Malgorzata.,Chlebowska, Justyna.,Nowis, Dominika.,Stachura, Joanna.,...&Golab, Jakub.(2014).Adenanthin targets proteins involved in the regulation of disulphide bonds.BIOCHEMICAL PHARMACOLOGY,89(2),210-216. |
| MLA | Muchowicz, Angelika,et al."Adenanthin targets proteins involved in the regulation of disulphide bonds".BIOCHEMICAL PHARMACOLOGY 89.2(2014):210-216. |
入库方式： OAI收割
来源：昆明植物研究所
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