Expression profiles of two small heat shock proteins and antioxidant enzyme activity in Mytilus galloprovincialis exposed to cadmium at environmentally relevant concentrations
文献类型：期刊论文
| 作者 | You Liping1,3; Ning Xuanxuan2; Chen Leilei1; Zhang Linbao1; Zhao Jianmin1 ; Liu Xiaoli1,2; Wu Huifeng1
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| 刊名 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
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| 出版日期 | 2014-03-01 |
| 卷号 | 32期号:2页码:334-343 |
| 关键词 | Mytilus galloprovincialis small heat shock protein superoxide dismutase (SOD) catalase (CAT) glutathione peroxidase (GPx) cadmium |
| ISSN号 | 0254-4059 |
| 产权排序 | [You Liping; Chen Leilei; Zhang Linbao; Zhao Jianmin; Liu Xiaoli; Wu Huifeng] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Processes & Ecol Re, Yantai 264003, Peoples R China; [Ning Xuanxuan; Liu Xiaoli] State Ocean Adm, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China; [You Liping] Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 通讯作者 | Zhao, JM (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Processes & Ecol Re, Yantai 264003, Peoples R China. jmzhao@yic.ac.cn ; hfwu@yic.ac.cn |
| 中文摘要 | Small heat shock proteins encompass a widespread but diverse class of proteins, which play key roles in protecting organisms from various stressors. In the present study, the full-length cDNAs of two small heat shock proteins (MgsHSP22 and MgsHSP24.1) were cloned from Mytilus galloprovincialis, which encoded peptides of 181 and 247 amino acids, respectively. Both MgsHSP22 and MgsHSP24.1 were detected in all tissues examined by real-time PCR, with the highest expression being observed in muscle and gonad tissues. The real-time PCR results revealed that Cd significantly inhibited MgsHSP22 expression at 24 h and MgsHSP24.1 at 24 and 48 h under 5 mu g/L Cd (2+) exposure. MgsHSP24.1 expression was also significantly inhibited after 50 mu g/L Cd2+ exposure for 48 h. With regard to antioxidant enzymes, increased GPx and CAT activity were detected under Cd2+ stress (5 and 50 mu g/L), while no significant difference in SOD activity was observed throughout the experiment. Overall, both MgsHsps and antioxidant enzymes revealed their potential as Cd stress biomarkers in M. galloprovincialis. |
| 英文摘要 | Small heat shock proteins encompass a widespread but diverse class of proteins, which play key roles in protecting organisms from various stressors. In the present study, the full-length cDNAs of two small heat shock proteins (MgsHSP22 and MgsHSP24.1) were cloned from Mytilus galloprovincialis, which encoded peptides of 181 and 247 amino acids, respectively. Both MgsHSP22 and MgsHSP24.1 were detected in all tissues examined by real-time PCR, with the highest expression being observed in muscle and gonad tissues. The real-time PCR results revealed that Cd significantly inhibited MgsHSP22 expression at 24 h and MgsHSP24.1 at 24 and 48 h under 5 mu g/L Cd (2+) exposure. MgsHSP24.1 expression was also significantly inhibited after 50 mu g/L Cd2+ exposure for 48 h. With regard to antioxidant enzymes, increased GPx and CAT activity were detected under Cd2+ stress (5 and 50 mu g/L), while no significant difference in SOD activity was observed throughout the experiment. Overall, both MgsHsps and antioxidant enzymes revealed their potential as Cd stress biomarkers in M. galloprovincialis. |
| 学科主题 | Limnology ; Oceanography |
| 研究领域[WOS] | Marine & Freshwater Biology ; Oceanography |
| 关键词[WOS] | INTRACELLULAR REDOX STATE ; ALPHA-CRYSTALLIN DOMAIN ; SMALL STRESS-PROTEINS ; OXIDATIVE STRESS ; METAL ACCUMULATION ; PERNA-VIRIDIS ; B-CRYSTALLIN ; CELL-DEATH ; HSP27 ; GLUTATHIONE |
| 收录类别 | SCI |
| 资助信息 | Chinese Academy of Sciences; National Natural Science Foundation of China [41206105]; Key Deployment Program of Chinese Academy of Sciences [KZZD-EW-14-03] |
| 原文出处 | http://dx.doi.org/10.1007/s00343-014-3111-9 |
| 语种 | 英语 |
| WOS记录号 | WOS:000332739600009 |
| 公开日期 | 2014-07-08 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/6897]  |
| 专题 | 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Processes & Ecol Re, Yantai 264003, Peoples R China 2.State Ocean Adm, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | You Liping,Ning Xuanxuan,Chen Leilei,et al. Expression profiles of two small heat shock proteins and antioxidant enzyme activity in Mytilus galloprovincialis exposed to cadmium at environmentally relevant concentrations[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2014,32(2):334-343. |
| APA | You Liping.,Ning Xuanxuan.,Chen Leilei.,Zhang Linbao.,Zhao Jianmin.,...&Wu Huifeng.(2014).Expression profiles of two small heat shock proteins and antioxidant enzyme activity in Mytilus galloprovincialis exposed to cadmium at environmentally relevant concentrations.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,32(2),334-343. |
| MLA | You Liping,et al."Expression profiles of two small heat shock proteins and antioxidant enzyme activity in Mytilus galloprovincialis exposed to cadmium at environmentally relevant concentrations".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 32.2(2014):334-343. |
入库方式： OAI收割
来源：烟台海岸带研究所
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