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Molecular cloning and expression of a C-type lectin gene from Venerupis philippinarum

文献类型：期刊论文

作者Mu, Changkao1; Chen, Leilei1,2,3; Zhao, Jianmin2,3; Wang, Chunlin1
刊名MOLECULAR BIOLOGY REPORTS
出版日期2014
卷号41期号:1页码:139-144
ISSN号0301-4851
关键词Venerupis philippinarum C-type lectin Gene expression
通讯作者Wang, CL (reprint author), Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China. wangchunlin@nbu.edu.cn
产权排序[Mu, Changkao; Chen, Leilei; Wang, Chunlin] Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China; [Chen, Leilei; Zhao, Jianmin] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Chen, Leilei; Zhao, Jianmin] Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
中文摘要C-type lectins have been demonstrated to play important roles in invertebrate innate immunity by mediating the recognition of pathogens and clearing the micro-invaders. In the present study, a C-type lectin gene (denoted as VpCTL) was identified from Venerupis philippinarum by expressed sequence tag and rapid amplification of cDNA ends approaches. The full-length cDNA of VpCTL consists of 904 nucleotides with an open-reading frame of 456 bp encoding a peptide of 151 amino acids. The deduced amino acid sequence of VpCTL shared high similarity with C-type lectins from other species. The C-type lectin domain and the characteristic EPN and WND motifs were found in VpCTL. The VpCTL mRNA was dominantly expressed in the haemocytes of the V. philippinarum. After Listonella anguillarum challenge, the temporal expression of VpCTL mRNA in haemocytes was increased by 97- and 84-fold at 48 and 96 h, respectively. With high expression level in haemocytes and hepatopancreas, and the up-regulated expression in haemocytes indicted that VpCTL was perhaps involved in the immune responses to L. anguillarum challenge.
英文摘要C-type lectins have been demonstrated to play important roles in invertebrate innate immunity by mediating the recognition of pathogens and clearing the micro-invaders. In the present study, a C-type lectin gene (denoted as VpCTL) was identified from Venerupis philippinarum by expressed sequence tag and rapid amplification of cDNA ends approaches. The full-length cDNA of VpCTL consists of 904 nucleotides with an open-reading frame of 456 bp encoding a peptide of 151 amino acids. The deduced amino acid sequence of VpCTL shared high similarity with C-type lectins from other species. The C-type lectin domain and the characteristic EPN and WND motifs were found in VpCTL. The VpCTL mRNA was dominantly expressed in the haemocytes of the V. philippinarum. After Listonella anguillarum challenge, the temporal expression of VpCTL mRNA in haemocytes was increased by 97- and 84-fold at 48 and 96 h, respectively. With high expression level in haemocytes and hepatopancreas, and the up-regulated expression in haemocytes indicted that VpCTL was perhaps involved in the immune responses to L. anguillarum challenge.
学科主题Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]SCALLOP ARGOPECTEN-IRRADIANS ; SHRIMP LITOPENAEUS-VANNAMEI ; CHLAMYS-FARRERI ; DOMAIN SUPERFAMILY ; RECOGNITION
资助信息Doctoral Fund of Ministry of Education of China [20103305110003]; National Key Technology Support Program [2011BAD13B0903]; K.C.Wong Magna Fund in Ningbo University
收录类别SCI
原文出处http://dx.doi.org/10.1007/s11033-013-2846-2
语种英语
WOS记录号WOS:000329100500017
公开日期2014-07-08
源URL[http://ir.yic.ac.cn/handle/133337/6922]  
专题烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位1.Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China
2.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
3.Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714		 Mu, Changkao,Chen, Leilei,Zhao, Jianmin,et al. Molecular cloning and expression of a C-type lectin gene from Venerupis philippinarum[J]. MOLECULAR BIOLOGY REPORTS,2014,41(1):139-144.
APA Mu, Changkao,Chen, Leilei,Zhao, Jianmin,&Wang, Chunlin.(2014).Molecular cloning and expression of a C-type lectin gene from Venerupis philippinarum.MOLECULAR BIOLOGY REPORTS,41(1),139-144.
MLA Mu, Changkao,et al."Molecular cloning and expression of a C-type lectin gene from Venerupis philippinarum".MOLECULAR BIOLOGY REPORTS 41.1(2014):139-144.

入库方式： OAI收割

来源：烟台海岸带研究所

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