Pulsed Galvanostatic Control of a Polymeric Membrane Ion-Selective Electrode for Potentiometric Immunoassays
文献类型：期刊论文
| 作者 | Ding, Jiawang1,2 ; Wang, Xuewei1; Qin, Wei1
|
| 刊名 | ACS APPLIED MATERIALS & INTERFACES
 |
| 出版日期 | 2013-10-09 |
| 卷号 | 5期号:19页码:9488-9493 |
| 关键词 | immunoassays potentiometry polymeric membrane electrodes pulsed galvanostatic technique sample-membrane interface |
| ISSN号 | 1944-8244 |
| 其他题名 | The Ecological Restoration of Heavily Degraded Saline Wetland in the Yellow River Delta.pdf |
| 产权排序 | [Ding, Jiawang; Wang, Xuewei; Qin, Wei] Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai Inst Coastal Zone Res YIC,YICCAS, Yantai 264003, Shandong, Peoples R China; [Ding, Jiawang] Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 通讯作者 | Qin, W (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, 17 Chunhui Rd, Yantai 264003, Shandong, Peoples R China. wqin@yic.ac.cn |
| 中文摘要 | Pulsed galvanostatic control of ion fluxes across polymeric membrane ion-selective electrodes (ISEs) is an emerging field for potentiometric sensing. Herein we report a novel potentiometric enzyme immunoassay based on current-controlled release of an enzyme substrate, which eliminates the addition of marker ions in the sample solution. In this method, the carboxylated poly(vinyl chloride) matrix at the outer layer of the ISE membrane is employed to attach a primary antibody. A sandwich immunoassay with an alkaline phosphatase labeled antibody (ALP-Ab) as the reporter is used for the determination of human IgG (as a model protein). The large difference between the lipophilicity of the substrate ion and that of the product ion allows p-nitrophenyl phosphate to be used as the enzyme substrate for potentiometric immunosensors. After the immunoreactions, the captured ALP-Ab catalyzes the hydrolysis of the substrate ions released at the sample-membrane interface by using the pulsed galvanostatic technique. This process can be potentiometrically determined by measuring the open circuit potential of the ISE. Under optimal conditions, the potential response of the proposed immunosensor is proportional to the concentration of human IgG in the range of 50-1000 ng/mL with a detection limit of 30 ng/mL (3 sigma). Owing to simplicity and independence of sample volume and sample turbidity, the proposed potentiometric immunoassay offers a viable alternative to those based on optical absorbance. |
| 英文摘要 | Pulsed galvanostatic control of ion fluxes across polymeric membrane ion-selective electrodes (ISEs) is an emerging field for potentiometric sensing. Herein we report a novel potentiometric enzyme immunoassay based on current-controlled release of an enzyme substrate, which eliminates the addition of marker ions in the sample solution. In this method, the carboxylated poly(vinyl chloride) matrix at the outer layer of the ISE membrane is employed to attach a primary antibody. A sandwich immunoassay with an alkaline phosphatase labeled antibody (ALP-Ab) as the reporter is used for the determination of human IgG (as a model protein). The large difference between the lipophilicity of the substrate ion and that of the product ion allows p-nitrophenyl phosphate to be used as the enzyme substrate for potentiometric immunosensors. After the immunoreactions, the captured ALP-Ab catalyzes the hydrolysis of the substrate ions released at the sample-membrane interface by using the pulsed galvanostatic technique. This process can be potentiometrically determined by measuring the open circuit potential of the ISE. Under optimal conditions, the potential response of the proposed immunosensor is proportional to the concentration of human IgG in the range of 50-1000 ng/mL with a detection limit of 30 ng/mL (3 sigma). Owing to simplicity and independence of sample volume and sample turbidity, the proposed potentiometric immunoassay offers a viable alternative to those based on optical absorbance. |
| 学科主题 | Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary |
| 研究领域[WOS] | Science & Technology - Other Topics ; Materials Science |
| 关键词[WOS] | ALKALINE-PHOSPHATASE ; ELECTROCHEMICAL IMMUNOASSAY ; IMMUNOSENSOR ; SENSOR ; IMMOBILIZATION ; COEFFICIENTS ; SUBSTRATE ; MATRICES ; HEPARIN ; ANTIGEN |
| 收录类别 | SCI |
| 资助信息 | Instrument Developing Project of the Chinese Academy of Sciences [YZ201161]; National Natural Science Foundation of China [41176081, 21207156]; Science and Technology Project of Yantai [2012132]; Taishan Scholar Program of Shandong Province [TS20081159] |
| 原文出处 | http://dx.doi.org/10.1021/am402245f |
| 语种 | 英语 |
| WOS记录号 | WOS:000326356600033 |
| 公开日期 | 2014-07-08 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/6944]  |
| 专题 | 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai Inst Coastal Zone Res YIC,YICCAS, Yantai 264003, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ding, Jiawang,Wang, Xuewei,Qin, Wei. Pulsed Galvanostatic Control of a Polymeric Membrane Ion-Selective Electrode for Potentiometric Immunoassays[J]. ACS APPLIED MATERIALS & INTERFACES,2013,5(19):9488-9493. |
| APA | Ding, Jiawang,Wang, Xuewei,&Qin, Wei.(2013).Pulsed Galvanostatic Control of a Polymeric Membrane Ion-Selective Electrode for Potentiometric Immunoassays.ACS APPLIED MATERIALS & INTERFACES,5(19),9488-9493. |
| MLA | Ding, Jiawang,et al."Pulsed Galvanostatic Control of a Polymeric Membrane Ion-Selective Electrode for Potentiometric Immunoassays".ACS APPLIED MATERIALS & INTERFACES 5.19(2013):9488-9493. |
入库方式： OAI收割
来源：烟台海岸带研究所
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