Proteomic and metabolomic analysis of earthworm Eisenia fetida exposed to different concentrations of 2,2 ',4,4 '-tetrabromodiphenyl ether
文献类型：期刊论文
| 作者 | Ji, Chenglong1,2,3 ; Wu, Huifeng1,2; Wei, Lei1,2,3; Zhao, Jianmin1,2; Lu, Hongjian4; Yu, Junbao1,2
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| 刊名 | JOURNAL OF PROTEOMICS
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| 出版日期 | 2013-10-08 |
| 卷号 | 91页码:405-416 |
| 关键词 | Proteomics Metabolomics Eisenia fetida BDE 47 |
| ISSN号 | 1874-3919 |
| 其他题名 | Synthesis and antifungal activity of thiadiazole-functionalized chitosan derivatives.pdf |
| 产权排序 | [Ji, Chenglong; Wu, Huifeng; Wei, Lei; Zhao, Jianmin; Yu, Junbao] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Ji, Chenglong; Wu, Huifeng; Wei, Lei; Zhao, Jianmin; Yu, Junbao] YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Ji, Chenglong; Wei, Lei] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China; [Lu, Hongjian] Nanjing Univ, Inst Chem & Biomed Sci ICBMS, Nanjing 210046, Jiangsu, Peoples R China |
| 通讯作者 | Wu, HF (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China. hfwu@yic.ac.cn |
| 中文摘要 | As a class of brominated flame-retardants (BFRs), polybrominated diphenyl ethers (PBDEs) are widely used in industrial products. PBDEs have been detected in local terrestrial biota from the Laizhou Bay in China. They can induce various toxiciides, such as hepatotoxicity, neurotoxicity, cytotoxicity, genotoxicity and endocrine disrupting effects in animals. In this work, we characterized the dose-responsive effects of 2,2',4,4'-tetrabromodiphenyl ether (BDE 47) in earthworrn Eisenia fetida using an integrated proteomic and metabolomic approach. Metabolic responses indicated that BDE 47 mainly caused disturbance in osmotic regulation and energy metabolism marked by differentially altered betaine, amino acids, ATP, glucose, maltose and succinate in E. fetida. Proteomic responses revealed that BDE 47 induced cell apoptosis (or injury), oxidative stress, disturbance in protein biosynthesis and energy metabolism in E. fetida in terms of differential proteomic biomarkers. Especially, the increased ATP was confirmed by up-regulated nucleoside diphosphate kinase A and ATP synthase in 1 and 100 mu g/L of BDE 47-treated groups, respectively. In addition, several metabolic biomarkers including betaine, glycine and 2-hexyl-5-ethyl-3-furansulfonate were relatively stable in all BIDE 47-exposed groups. This work demonstrated that proteomics and metabolomics could partially validate one another and their combination could better understand toxicological effects of environmental pollutants. Biological significance As a class of brominated flame-retardants (BFRs), polybrominated diphenyl ethers (PBDEs) are widely used in industrial products and have been detected in local terrestrial biota from the Laizhou Bay in China. Therefore a study on PBDE-induced toxicological effects is necessary. The earthworm E. fetida, a terrestrial sentinel animal, is the most frequently used bioindicator for terrestrial environmental contaminants. To our knowledge, however, very few studies have focused on the dose-dependent responses induced by PBDEs, in terrestrial sentinel animal at protein and metabolite levels. In the present study, an integrated metabolomic and proteomic approach was used to elucidate the dose-dependent toxicological effects of BIDE 47 in E. fetida. (C) 2013 Elsevier B.V. All rights reserved. |
| 英文摘要 | As a class of brominated flame-retardants (BFRs), polybrominated diphenyl ethers (PBDEs) are widely used in industrial products. PBDEs have been detected in local terrestrial biota from the Laizhou Bay in China. They can induce various toxiciides, such as hepatotoxicity, neurotoxicity, cytotoxicity, genotoxicity and endocrine disrupting effects in animals. In this work, we characterized the dose-responsive effects of 2,2',4,4'-tetrabromodiphenyl ether (BDE 47) in earthworrn Eisenia fetida using an integrated proteomic and metabolomic approach. Metabolic responses indicated that BDE 47 mainly caused disturbance in osmotic regulation and energy metabolism marked by differentially altered betaine, amino acids, ATP, glucose, maltose and succinate in E. fetida. Proteomic responses revealed that BDE 47 induced cell apoptosis (or injury), oxidative stress, disturbance in protein biosynthesis and energy metabolism in E. fetida in terms of differential proteomic biomarkers. Especially, the increased ATP was confirmed by up-regulated nucleoside diphosphate kinase A and ATP synthase in 1 and 100 mu g/L of BDE 47-treated groups, respectively. In addition, several metabolic biomarkers including betaine, glycine and 2-hexyl-5-ethyl-3-furansulfonate were relatively stable in all BIDE 47-exposed groups. This work demonstrated that proteomics and metabolomics could partially validate one another and their combination could better understand toxicological effects of environmental pollutants. |
| 学科主题 | Biochemical Research Methods |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | POLYBROMINATED DIPHENYL ETHERS ; H295R CELL-LINE ; PBDE METABOLITES ; PROTEINS ; IDENTIFICATION ; STRESS ; GENE ; MUSCLE ; STEROIDOGENESIS ; ENVIRONMENT |
| 收录类别 | SCI |
| 资助信息 | 100 Talents Program of the Chinese Academy of Sciences; Key Deployment Program of Chinese Academy of Sciences [KZZD-EW-14-03] |
| 原文出处 | http://dx.doi.org/10.1016/j.jprot.2013.08.004 |
| 语种 | 英语 |
| WOS记录号 | WOS:000327906000032 |
| 公开日期 | 2014-07-08 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/6945]  |
| 专题 | 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 烟台海岸带研究所_滨海湿地实验室 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China 2.YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 4.Nanjing Univ, Inst Chem & Biomed Sci ICBMS, Nanjing 210046, Jiangsu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ji, Chenglong,Wu, Huifeng,Wei, Lei,et al. Proteomic and metabolomic analysis of earthworm Eisenia fetida exposed to different concentrations of 2,2 ',4,4 '-tetrabromodiphenyl ether[J]. JOURNAL OF PROTEOMICS,2013,91:405-416. |
| APA | Ji, Chenglong,Wu, Huifeng,Wei, Lei,Zhao, Jianmin,Lu, Hongjian,&Yu, Junbao.(2013).Proteomic and metabolomic analysis of earthworm Eisenia fetida exposed to different concentrations of 2,2 ',4,4 '-tetrabromodiphenyl ether.JOURNAL OF PROTEOMICS,91,405-416. |
| MLA | Ji, Chenglong,et al."Proteomic and metabolomic analysis of earthworm Eisenia fetida exposed to different concentrations of 2,2 ',4,4 '-tetrabromodiphenyl ether".JOURNAL OF PROTEOMICS 91(2013):405-416. |
入库方式： OAI收割
来源：烟台海岸带研究所
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